Methods of Protein Microcharacterization
Language: English
Published by Humana Press, Humana Press Jul 1986, 1986
- Hardcover
- New
Seller: buchversandmimpf2000, Emtmannsberg, BAYE, Germanybuchversandmimpf2000
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This item is printed on demand - Print on Demand Titel. Neuware -Milestones in the techniques and methodology of polypeptide structure determination include the determination of the sequence of insulin by Sanger in 1951 (I) and the introduction of the repeti tive degradation of proteins with phenylisothiocyanate by Edman in 1959 (2). The automation of Edman chemistry (3) played a major role in the determination of polypeptide structures. Important modifications of Edman chemistry include the solid-phase approach by Laursen in 1971 (4) and the use of modified Edman reagents such as 4-N,N-dimethylaminoazobenzene-4'-isothiocy- ate (DABITC) for manual sequencing by Chang et al. (5) in 1976. A second major breakthrough in the analysis of polypeptides was automated amino acid analysis described by Spackman et al. in 1958 (6). However, during the period from 1975 to 1980, it became increasingly clear that the amount of material required for struc tural analysis was more than could be easily isolated for the vast majority of proteins. The field was criticized for its lack of sensitive techniques for the analysis of growth factors, immune modulators, membrane receptors, and peptide hormones. In addition, very little had been done to modernize and improve the original instruments introduced in the mid-1960s. The first indications of improved instrumentation for Edman chemistry came from Wittmann-Liebold's laboratory (7), followed by the introduction of a 'micro' sequencer by Hunkapiller and Hood in 1978 (8). The movement toward improved instrumentation culminated in the 'gas'-phase sequencer of Hewick et al. (9) in 1981.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 480 pp. Englisch.…
Seller Inventory # 9780896030909
- Title
- Methods of Protein Microcharacterization
- Author
- John E. Shively
- Publisher
- Humana Press, Humana Press Jul 1986
- Publication year
- 1986
- Condition
- Neu
- Binding
- Buch
- Language
- English
- ISBN 10
- 0896030903
- ISBN 13
- 9780896030909
- Item weight
- 842 grams
- Dimensions
- 235x157x31 mm
"Synopsis" may belong to another edition of this title.
buchversandmimpf2000
Emtmannsberg, BAYE, Germany
AbeBooks seller since January 23, 2017
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