This book highlights recent advances in and diverse techniques for exploring the plasma membrane’s structure and function. It starts with two chapters reviewing the history of membrane research and listing recent advances regarding membrane structure, such as the semi-mosaic model for red blood cell membranes and the protein layer-lipid-protein island model for nucleated tissue cell membranes. It subsequently focuses on the localization and interactions of membrane components, dynamic processes of membrane transport and transmembrane signal transduction. Classic and cutting-edge techniques (e.g. high-resolution atomic force microscopy and super-resolution fluorescence microscopy) used in biophysics and chemistry are presented in a very comprehensive manner, making them useful and accessible to both researchers in the field and novices studying cell membranes. This book provides readers a deeper understanding of the plasma membrane’s organization at the single molecule level and opens a new way to reveal the relationship between the membrane’s structure and functions, making it essential reading for researchers in various fields.

About the Author

Hongda Wang received his bachelor’s degree from the Department of Molecular Biology, Jilin University in 1995 and his doctorate from the Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, in 2000. He worked as a postdoctoral research associate at the Max Planck Institute of Molecular Physiology and Arizona State University from 2001 to 2007. Since 2008, he has been working as a PI at the Changchun Institute of Applied Chemistry (under the CAS 100 Talent Program). Prof. Wang is the winner of The National Science Fund for Distinguished Young Scholars. His primary research interest is in the structure and function of cell membranes using multiple single-molecule techniques, particularly atomic force microscopy, molecular recognition imaging and super-resolution fluorescence microscopy.

Guohui Li received his bachelor’s degree from the Department of Physics, Liaoning Normal University in 1994 and his doctorate from Dalian Institute of Chemical Physics(DICP), Chinese Academy of Sciences, in 2000. He worked as a postdoctoral research associate at the University of New Mexico, University of Wisconsin Madison, and Harvard Medical School from 2000 to 2006, and served as a research scientist at Biogen Idec Inc. from 2006 to 2009. Since 2009, he has been working as a PI at DICP. His main research interest is in studying the biophysical properties and biochemical functionality of soluble and membrane-associated macromolecules through computational simulation techniques; the developments in and applications of force fields with high precision and efficiency; and the combination of new force fields and enhanced sampling techniques. The polarizable lipid models developed by his lab have been used to study the structure, dynamics of membrane bilayers, and microscopic mechanism of membrane proteins.

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