Mast Cells: Methods and Protocols (Methods in Molecular Biology, 315)

Krishnaswamy, Guha [Editor]; Chi, David S. [Editor];

ISBN 10: 1588293742 / ISBN 13: 9781588293749

Published by Humana, 2005

2005, First Printing with full number line. Binding is tight, sturdy, and square; minor wear to edges of glossy boards; text also very good. Ships from Dinkytown in Minneapolis, Minnesota. Seller Inventory # 294360

Synopsis:

A cutting-edge collection of readily reproducible techniques for the isolation, culture, and study of activation and signaling in human mast cells. These methods take advantage of the latest advances in molecular biology, technology, and information science. They include methods for the identification of mast cells, the development of mast cells in vitro, the study of mast cell signaling and gene expression, and the measurement of mast cell expression of inflammatory mediators. Additional chapters cover methods for studying mast cell interactions with other cell types (endothelial cells, fibroblasts, and B cells), the roles of mast cells in host defense, and mast cell apoptosis.

From the Back Cover: The mast cell, long implicated in causing allergic reactions, may also be involved in many other disease processes, including cancer, heart disease, parasitic disease, atherosclerosis, asthma, and arthritis. In Mast Cells: Methods and Protocols, hands-on experts describe in detail their best techniques for the isolation, culture, and study of both activation and signaling in human mast cells. These readily reproducible methods take advantage of the latest advances in molecular biology, technology, and information science. The techniques provide a sound base of methodology for mast cell research and include methods for the identification of mast cells, the development of mast cells in vitro, the study of mast cell signaling and gene expression, and the measurement of mast cell expression of inflammatory mediators. Additional chapters cover methods for studying mast cell interactions with other cell types (endothelial cells, fibroblasts, and B cells), the roles of mast cells in host defense, and mast cell apoptosis. A survey of mast cell biology offers insight into its history and the implications for adaptive immunity. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and highly practical, Mast Cells: Methods and Protocols provides mast cell researchers with reproducible accounts of basic and advanced molecular and cellular techniques used in studying this fascinating, multifunctional cell.

Bibliographic Details

Title: Mast Cells: Methods and Protocols (Methods ...
Publisher: Humana
Publication Date: 2005
Binding: Hardcover
Condition: Very Good