Kinase C Probes : Fluorescent Sensors for Dynamic Intracellular Signalling

Language: English

Published by VDM Verlag Dr. Müller, VDM Verlag Dr. Müller E.K., 2008

3639036514 / 9783639036510

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Seller: AHA-BUCH GmbH, Einbeck, GermanyAHA-BUCH GmbH

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nach der Bestellung gedruckt Neuware - Printed after ordering - Understanding cellular function is a pivotal aim in the life sciences. Monitoring the interplay of regulatory proteins illuminates cellular function on a molecular level. One powerful method is fluorescence microscopy, which enables the spatio-temporal resolution of cellular events. To this end, several fluorescent reporters can be monitored within a single cell and interdependency between these factors can be determined.This book presents fluorescent reporters for monitoring protein kinase C, a central actor on the stage of cellular signalling. Aspects ranging from probe design to the evaluation of experimental results are discussed. Further, experiments designed for high-throughput screening of new probe variants or PKC-effector molecules are described, including methods for visualizing and judging the observed data. Finally, the probe's capacity for use in multiparameter experiments is demonstrated. This book is complemented by a detailed experimental part including a flexible cloning system for rapid probe development and computer scripts supporting the analysis of experimental data.This book is directed to researchers interested in fluorescence probe development.…

Seller Inventory # 9783639036510

Title
Kinase C Probes : Fluorescent Sensors for Dynamic Intracellular Signalling
Author
Andreas Schleifenbaum
Publisher
VDM Verlag Dr. Müller, VDM Verlag Dr. Müller E.K.
Publication year
2008
Condition
Neu
Binding
Taschenbuch
Language
English
ISBN 10
3639036514
ISBN 13
9783639036510
Item weight
249 grams
Dimensions
220x150x9 mm

AHA-BUCH GmbH

Einbeck, Germany

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