Insect Genomics : Methods and Protocols

Michael E. Pfrender

ISBN 10: 1493993895 / ISBN 13: 9781493993895

Published by Springer New York, Springer New York, 2019

About this Item

Druck auf Anfrage Neuware - Printed after ordering - This volume focuses on the latest methods used to sequence, assemble, and analyze insect genomes. The collection of protocols in this book provides an introduction to the workflows and bioinformatics tools available for researchers. The chapters cover a range of useful topics such as determining genome size by flow cytometry; High Molecular Weight DNA extraction; improvements to a genome assembly provided by long-range sequencing approaches; assessments of orthology and single-copy genes at different phylogenetic levels; detecting regulatory regions with FAIRE, RAMPAGE, and computational analysis of cis-regulatory modules in insects; bioinformatics analysis of epigenetic modifications, high-throughput scanning of insect genomes (TEEseq) for the presence of endosymbionts, and leveraging genome sequence information to design RNAi strategies. Written in the highly successfulMethods in Molecular Biologyseries format, chapters include introductions to their respectivetopics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough,Insect Genomics: Methods and Protocolsis a valuable resource for graduate students, postdocs, and novice research scientists who are interested in learning more about this developing field.

Seller Inventory # 9781493993895

Bibliographic Details

• Title: Insect Genomics : Methods and Protocols
• Publisher: Springer New York, Springer New York
• Publication Date: 2019
• Language: English
• ISBN 10: 1493993895
• ISBN 13: 9781493993895
• Binding: Taschenbuch
• Condition: Neu
• Dimensions: 254W x 178H x 14D millimeters 254 millimeters width by 178 millimeters height by 14 millimeters depth
• Weight: 482 grams

About this title

Synopsis

This volume focuses on the latest methods used to sequence, assemble, and analyze insect genomes. The collection of protocols in this book provides an introduction to the workflows and bioinformatics tools available for researchers. The chapters cover a range of useful topics such as determining genome size by flow cytometry; High Molecular Weight DNA extraction; improvements to a genome assembly provided by long-range sequencing approaches; assessments of orthology and single-copy genes at different phylogenetic levels; detecting regulatory regions with FAIRE, RAMPAGE, and computational analysis of cis-regulatory modules in insects; bioinformatics analysis of epigenetic modifications, high-throughput scanning of insect genomes (TEEseq) for the presence of endosymbionts, and leveraging genome sequence information to design RNAi strategies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respectivetopics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Insect Genomics: Methods and Protocols is a valuable resource for graduate students, postdocs, and novice research scientists who are interested in learning more about this developing field.