Get familiar with one of the world's most highly regarded Digital Image processors, ImageJ. This tutorial takes you through every aspect of viewing, processing, and analysing 2D, 3D, and 4D images, clearly and comprehensively.
Overview
- Learn how to process digital images using ImageJ and deal with a variety of formats and dimensions, including 4D images
- Understand what histograms, region of interest, or filtering means and how to analyze images easily with these tools
- Packed with practical examples and real images, with step-by-step instructions and sample code
In Detail
Digital image processing is an increasingly important field across a vast array of scientific disciplines. ImageJ’s long history and ever-growing user base makes it a perfect candidate for solving daily tasks involving all kinds of image analysis processes.
Image Processing with ImageJ is a practical book that will guide you from the most basic analysis techniques to the fine details of implementing new functionalities through the ImageJ plugin system, all of it through the use of examples and practical cases.
ImageJ is an excellent public domain imaging analysis platform that can be very easily used for almost all your image processing needs. Image Processing with ImageJ will start by showing you how to open a number of different images, become familiar with the different options, and perform simple analysis operations using the provided image samples.
You will also learn how to make modifications through ImageJ filters and how to make local measurements using the selections system. You will also find the instructions necessary to record all the steps you perform so they can be saved and re-run on the same image to ensure analysis reproducibility. Finally, you will get to know some different ImageJ plugins and will learn how to implement your own.
What you will learn from this book
- Install and configure ImageJ and its plugins
- Open different image formats
- Move around the image, zoom in and out, and obtain pixel values
- Master the different selection tools
- Plot the image profile and understand the histograms
- Handle 3D and 4D images
- Use the macro recording system
- Create your own macros beyond the recorder
- Build your own plugins using the ImageJ editor
- Integrate ImageJ with the Eclipse IDE to improve the plugin development process
Approach
The book will help readers discover the various facilities of ImageJ through a tutorial-based approach.
Who this book is written for
This book is targeted at scientists, engineers, technicians, and managers, and anyone who wishes to master ImageJ for image viewing, processing, and analysis. If you are a developer, you will be able to code your own routines after you have finished reading this book. No prior knowledge of ImageJ is expected.
Jose Maria Mateos Perez is a Spanish researcher and Ph.D. student in the Medical Imaging Laboratory of the Hospital General Universitario Gregorio Maranon (http://image.hggm.es), in Madrid, where his main research lines deal with automatic segmentation and kinetic analysis modeling of dynamic nuclear imaging sequences. He is an experienced ImageJ user and has developed several macros and plugins. One of them, jClustering, has been published in PLOS ONE, a peer-reviewed journal. When he has enough time to procrastinate, he also likes to develop data analysis tools in Python and R. Javier Pascau received his Ph.D. from the Polytechnic University in Madrid in 2006, and is currently a visiting professor at Carlos III University in Madrid. He is part of Biomedical Imaging and Instrumentation Group, a research laboratory with a multidisciplinary team of engineers, physicists, biologists, and physicians located both in the University and Hospital General Universitario Gregorio Maranon in Madrid (http://www.biig.uc3m.es). His research and teaching covers areas like medical image processing, analysis, quantification, and multimodal registration, both in preclinical and clinical environments. He has been involved in the development of small animal PET and CT devices, and in the last years he has led several projects on intraoperative radiation therapy and image guided surgery. He is the author of more than 30 papers published in peer-reviewed journals over the last 13 years.
