Guide to Techniques in Mouse Development, Part B, is an authoritative guide to different methods used in enzymology, focusing on investigating mouse development using technological advances. The text provides information regarding the principles of the methods in mouse development, and it offers readers reliable experimental protocols and recipes described comprehensively by leaders in the field of enzymology.

The text is divided into three sections and organized into 25 chapters. Below are several concepts covered by the text:

Lentivirus transgenesis o Germline modification using mouse stem cells
Electroporation
Applications of transposons in mouse genetics
Functional genomics using transposon systems
The use of DNA transposons in detecting cancer genes in mice
Recombination, conditional mutagenesis and induction of tamoxifen
Genetic fate mapping using recombinases
Genetic screens mouse ES cells
Gene trap mutagenesis
Mouse mutagenesis
Self- renewal and pluripotency
Transgenic RNAi applications
Gene knockdowns
Tetracycline-controlled transcription
Gene expression profiling of mouse embryos

The book is a comprehensive guide for students and professionals in genetics, cytology and molecular biology, who will find this book invaluable for their learning and practice.

Update of volume 225, Guide to Techniques in Mouse Development, edited by P.M. Wassarman and M.L. DePamphilis and published in 1993
Comprehensively covers:

new techniques for the cryopreservation of gametes and embryos
production of transgenic and null (knockout) animals (use of ES cells)
generation of conditional/inducible mutant animals
use of gene-trap mutagenesis
analysis of allele-specific expression
use of new reporter constructs
humanizing of transgenic animals
transcript profiling of mouse development
imaging of mouse development
rederivation of animals and use of mouse genomics

About the Author

Philippe Soriano,is Professor of Cell, Developmental & Regenerative Biology and Professor of Oncological Sciences, Icahn School of Medicine at Mount Sinai, NY, USA

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