A Green Fluorescent Protein-Based Purification System for Plant Cells : Optimizing Yield and Recovery of Recombinant Proteins from Plants
Language: English
Published by VDM Verlag Dr. Müller, 2009
- Softcover
- New
Seller: AHA-BUCH GmbH, Einbeck, GermanyAHA-BUCH GmbH
AbeBooks seller since August 14, 2006
Condition: New
£ 74.75
Quantity: 2 available
Add to basketItem description from seller
nach der Bestellung gedruckt Neuware - Printed after ordering - Purifying recombinant proteins from plants is often met with difficulties due to the complexity of plant cell extracts. Issues include high levels of protease activity, tannin and phenolic reactivity and hydrophobic aggregation. Difficulties in extraction and purification are major hurdles preventing the routine use of plants and plant suspension cells as bioreactors. Reporter molecules, such as green fluorescent protein, are often used to monitor recombinant protein expression levels. Therefore, a purification strategy was developed taking advantage of this commonly used reporter molecule. Although previous methods have been developed for purifying GFP, these strategies often included exposure to protein-denaturing solvents or required gel filtration and protein concentrating steps. This work details a strategy that optimizes yield and purity without jeopardizing the integrity of the fusion protein.
Seller Inventory # 9783639126426
- Title
- A Green Fluorescent Protein-Based Purification System for Plant Cells : Optimizing Yield and Recovery of Recombinant Proteins from Plants
- Author
- Gabriel Peckham
- Publisher
- VDM Verlag Dr. Müller
- Publication year
- 2009
- Condition
- Neu
- Binding
- Taschenbuch
- Language
- English
- ISBN 10
- 3639126424
- ISBN 13
- 9783639126426
- Item weight
- 237 grams
- Dimensions
- 220x176x33 mm
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