Genetic Modification Of Brassica Juncea: Agrobacterium Mediated Transformation Of Brassica Juncea With Antimicrobial Wasabi Defensin Gene

Khan Bangash, Sajid Ali; Khattak, Sahir Hameed; Swati, Zahoor Ahmad; Khan Bangash, Sajid Ali; Khattak, Sahir Hameed; Swati, Zahoor Ahmad

ISBN 10: 3846539171 / ISBN 13: 9783846539170

Published by Lap Lambert Academic Publishing, 2011

60 pages. 8.66x5.91x0.14 inches. In Stock. Seller Inventory # __3846539171

Synopsis:

Brassica juncea is one of the most important oilseed crop. We have developed an efficient and reproducible protocol for Agrobacterium-mediated transformation of B. juncea variety NIFA RAYA with Wasabi defensin gene to produce transgenic plants. This gene was isolated from the leaves of Wasabia japonica. We find out that co-cultivation of explants with the transformed agrobacterium inoculum having an OD ranged from 0.6-1 for 15-20 minutes give high transformation efficiency. These explants were then regenerated on MS medium containing 1-6 mgL-1 BAP, 0.1-0.6 mgL-1 NAA and 300 mgL-1 cefotoxim sodium with subsequent application of 10-100 mgL-1 kanamycin. Our results indicated that medium containing 3 mgL-1 BAP and 0.3 mgL-1 NAA was found to be the best hormone combination for callus formation whereas BAP 3 mgL-1 and NAA 0.5 mgL-1 was found to be the best combination for shoot regeneration. In all cases supplementing the medium with 20 μM AgNO3 had positive effect on callus formation and shoot regeneration. The transgenic nature of the transformed callus and regenerated shoots was confirmed via PCR analysis.

About the Author: Sajid Ali Khan Bangash, BSc (Hons)Plant breeding and genetics(silver medalist, M.Phil Biotechnology, Institute of Biotechnology and Genetic Engineering, Khyber Pakhtunkhwa Agricultural University Peshawar, Pakistan.

Bibliographic Details

Title: Genetic Modification Of Brassica Juncea: ...
Publisher: Lap Lambert Academic Publishing
Publication Date: 2011
Binding: Paperback
Condition: Brand New