Gene Targeting & Embryonic Stem Cells
Mcwhir, Jim; Thomson, Alison
ISBN 10: 1859963609 ISBN 13: 9781859963609
Published by Taylor & Francis, 2004
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Soft cover
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Synopsis:
Gene Targeting and Embryonic Stem Cells is a practical guide designed for the rapidly growing number of researchers who are moving into this field. Provides details on how to culture, transfect and differentiate established cell lines, and how to isolate new cell lines. Gene targeting experiments are described for a number of cell types, including ungulate fetal fibroblasts, murine ES cells, human embryonal carinoma cells and human ES cells, and include protocols for gene-targeting vectors, DNA transfection and RNA interference. The recent isolation of human embryonic stem cells and the potential of these cells for therapeutic applications has generated an entirely new methodology. Similarly, gene targeting methodology has recently been extended to nuclear donor cells in ungulate species. This volume will be invaluable for both new and established researchers in the field of human embryonic stem cells, and to biotech companies engaged in the production of transgenic proteins in livestock, xenotransplantation and the development of animal models.
About the Author: Jim McWhir (The Roslin Institute, UK) (Author) , Alison Thomson (The Roslin Institute, UK) (Author)
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Bibliographic Details
Title: Gene Targeting & Embryonic Stem Cells
Publisher: Taylor & Francis
Publication Date: 2004
Binding: Soft cover
Condition: New