Experiments in Biochemistry This second edition features new and revised experiments, including a new experiment that involves cloning the barracuda LDH gene, and new and expanded problem sets. Full description

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Introduction to the Text. Objectives of the Biochemistry Laboratory. Chapter Format of Hands on Biochemistry. 1. Biochemistry Boot Camp. 2. Acids, Bases, and Buffers. 3. Spectrophotometry. 4. Enzyme Purification. 5. Ion Exchange Chromatography. 6. Affinity Chromatography. 7. Gel filtration Chromatography. 8. Enzyme Kinetics. 9. Electrophoresis. 10. Western Blots. 11. Restriction Enzymes. 12. Cloning and Expression of Foreign Proteins. 13. Polymerase Chain Reaction.

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