Electron Probe Microanalysis : Applications in Biology and Medicine

Language: English

Published by Springer Berlin Heidelberg, 2011

3642744796 / 9783642744792

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Druck auf Anfrage Neuware - Printed after ordering - The aim of electron probe microanalysis of biological systems is to identify, localize, and quantify elements, mass, and water in cells and tissues. The method is based on the idea that all electrons and photons emerging from an electron beam irradiated specimen contain information on its structure and composition. In particular, energy spectroscopy of X-rays and electrons after interaction of the electron beam with the specimen is used for this purpose. However, the application of this method in biology and medicine has to overcome three specific problems: 1. The principle constituent of most cell samples is water. Since liquid water is not compatible with vacuum conditions in the electron microscope, specimens have to be prepared without disturbing the other components, in parti cular diffusible ions (elements). 2. Electron probe microanaly sis provides physical data on either dry specimens or fully hydrated, frozen specimens. This data usually has to be con verted into quantitative data meaningful to the cell biologist or physiologist. 3. Cells and tissues are not static but dynamic systems. Thus, for example, microanalysis of physiolo gical processes requires sampling techniques which are adapted to address specific biological or medical questions. During recent years, remarkable progress has been made to overcome these problems. Cryopreparation, image analysis, and electron energy loss spectroscopy are key areas which have solved some problems and offer promise for future improvements.…

Seller Inventory # 9783642744792

Title
Electron Probe Microanalysis : Applications in Biology and Medicine
Author
Karl Zierold
Publisher
Springer Berlin Heidelberg
Publication year
2011
Condition
Neu
Binding
Taschenbuch
Language
English
ISBN 10
3642744796
ISBN 13
9783642744792
Item weight
517 grams
Dimensions
235x155x19 mm

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