Electrochemical synchronization of cell spreading on solid surfaces

Language: English

Published by LAP LAMBERT Academic Publishing Okt 2010, 2010

3843360634 / 9783843360630

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This item is printed on demand - Print on Demand Titel. Neuware -Cell synchronization is important for the analysis of molecular events involved in cell spreading and motility. Electrostatic interactions between cells and surfaces were investigated in order to synchronize the first step in cell adhesion. LimE- GFP marked Dictyostelium discoideum cells were used for fluorescent tracking of actin polymerization events. Oscillating LimE fluorescent peaks were observed for individual cells in standard phosphate buffer during spreading. At low ionic concentration (phosphate sucrose buffer 0.17 mM), cells levitate over the conductive surfaces (Indium Tin Oxide, ITO) due to electrostatic repulsion. An electrochemical device was designed in order to apply an overpotential pulse (+2.5 V/Ag,AgCl) during 0.1 s to the ITO surface. In these conditions, protons are produced by water oxidation, which reduce the ITO negative surface charge and thus, attracting the levitating cells simultaneously. Consequently, these irreversible contacts with the surface triggered the onset of cell spreading. Therefore, we obtained synchronization of the spreading of a cell population for the first time thanks to an electrochemical method.VDM Verlag, Dudweiler Landstraße 99, 66123 Saarbrücken 168 pp. Englisch. …

Seller Inventory # 9783843360630

Title
Electrochemical synchronization of cell spreading on solid surfaces
Author
Marius Socol
Publisher
LAP LAMBERT Academic Publishing Okt 2010
Publication year
2010
Condition
Neu
Binding
Taschenbuch
Language
English
ISBN 10
3843360634
ISBN 13
9783843360630
Item weight
268 grams
Dimensions
220x150x11 mm

buchversandmimpf2000

Emtmannsberg, BAYE, Germany

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