Drought-responsive transcript(s) profiling from the leaves of soybean

Language: English

Published by LAP LAMBERT Academic Publishing Sep 2012, 2012

3659212482 / 9783659212482

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This item is printed on demand - Print on Demand Titel. Neuware -Drought responsive genes or transcription factors are important components of any genetic engineering or breeding approach to enhance drought tolerance trait of a crop.Water-deficit stress was imposed on 30 days old seedlings of soybean cultivars Hardee and NRC7.Drought associated physiological parameter like relative water content revealed that water status of the stressed plants is low. Similarly studies on membrane damage disclosed plants under moisture-deficit stress exhibited higher electrolytic leakages, hence greater membrane damage.Total RNA isolated from both the control and stressed plant leaves of cultivars Hardee and NRC-7 was subjected to cDNA synthesis using 3 different anchored primers and thus mRNA population is sub-divided in to 3 different cDNA populations.DDRTPCR using the same anchored primers in combination with eight different arbitrary primers exhibited the transcript profiling of cultivars under moisture deficit stress.The transcript profiling was resolved in denaturing sequencing gel PAGE followed by silver staining so as to identify the differentially expressed transcripts.The book sheds light on drought responsive molecular mechanism of soybean.VDM Verlag, Dudweiler Landstraße 99, 66123 Saarbrücken 108 pp. Englisch.…

Seller Inventory # 9783659212482

Title
Drought-responsive transcript(s) profiling from the leaves of soybean
Author
Priti Roy
Publisher
LAP LAMBERT Academic Publishing Sep 2012
Publication year
2012
Condition
Neu
Binding
Taschenbuch
Language
English
ISBN 10
3659212482
ISBN 13
9783659212482
Item weight
179 grams
Dimensions
220x150x8 mm

buchversandmimpf2000

Emtmannsberg, BAYE, Germany

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