Development of recombinant UL30 protein of duck enteritis virus
Aravind S. Pillai (u. a.)
ISBN 10: 3659628670 ISBN 13: 9783659628672
Published by LAP LAMBERT Academic Publishing, 2014
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Development of recombinant UL30 protein of duck enteritis virus | Aravind S. Pillai (u. a.) | Taschenbuch | 88 S. | Englisch | 2014 | LAP LAMBERT Academic Publishing | EAN 9783659628672 | Verantwortliche Person für die EU: preigu GmbH & Co. KG, Lengericher Landstr. 19, 49078 Osnabrück, mail[at]preigu[dot]de | Anbieter: preigu. Seller Inventory # 104991766
Synopsis:
Duck viral enteritis, is caused by duck enteritis virus (DEV), is an acute, contagious and lethal disease. DEV is currently classified to belong to the alphaherpesvirinae subfamily of the herpesviridae family. The DNA polymerase, a product of UL30, is of central importance for successful viral replication in alpha herpes virinae group of viruses. The viral enzyme is involved in the initiating events of gene amplification processes. The UL30 protein is highly conserved among herpesviruses at the amino acid sequence level. This is one of the important protein for the replication of virus in the host cell. The DNA sequences encoding UL30 was identified from the genomic DNA of DEV. In this work we developed a recombinant UL30 protein of DEV from a prokaryotic system. The expressed recombinant protein obtained as a fusion protein with histidine tag was purified by affinity chromatography. The purified recombinant protein was confirmed by SDS-PAGE and western blot analysis using anti DEV sera which detected an 80 kDa protein band in the blot. The recombinant protein was further dialysed and concentrated using Amicon filters and the total yield of the protein was found to be 120µg/ml.
About the Author: Dr. Aravind S. Pillai,completed his Bachelor degree in Veterinary Science (BVSc & AH) from Kerala Agricultural University, Thrissur. And has done his Masters, (MVSc) and Ph.D. degrees in Animal Biotechnology from Indian Veterinary Research Institute, Izatnagar, Bareilly, UP.
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Bibliographic Details
Title: Development of recombinant UL30 protein of ...
Publisher: LAP LAMBERT Academic Publishing
Publication Date: 2014
Binding: Taschenbuch
Condition: Neu