Development of a Fully Integrated \ Sample-In-Answer-Out\ System for Automatic Genetic Analysis
Language: English
Published by Springer Singapore, 2017
- Hardcover
- New
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Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. Nominated by Tsinghua University as an outstanding Ph.D. thesisDevelops a disposable DNA extraction system and PCR microchip, as well as a compact control instrument for genetic testing of hereditary hearing loss from human whole blood with an imp.
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- Title
- Development of a Fully Integrated \ Sample-In-Answer-Out\ System for Automatic Genetic Analysis
- Author
- Bin Zhuang
- Publisher
- Springer Singapore
- Publication year
- 2017
- Condition
- New
- Binding
- Gebunden
- Language
- English
- ISBN 10
- 9811047529
- ISBN 13
- 9789811047527
- Series
- Book 306 of 797: Springer Theses
This thesis reports on the development of a fully integrated and automated microsystem consisting of low-cost, disposable plastic chips for DNA extraction and PCR amplification, combined with a reusable glass capillary array electrophoresis chip, which can be employed in a modular-based format for genetic analysis. In the thesis, DNA extraction is performed by adopting a filter paper-based method, followed by an “in-situ” PCR carried out directly in the same reaction chamber of the chip without elution. PCR products are then co-injected with sizing standards into separation channels for detection using a novel injection electrode. The entire process is automatically carried out by a custom-made compact control and detection instrument. The author thoroughly tests the system’s performance and reliability by conducting rapid genetic screening of mutations on congenital hearing loss and pharmacogenetic typing of multiple warfarin-related single-nucleotide polymorphisms. The successful development and operation of this microsystem establishes the feasibility of rapid “sample-in-answer-out” testing in routine clinical practice.
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About the Author
For internal review:
Dr. Bin Zhuang
Supervisor: Prof. Jing Cheng, School of Medicine, Tsinghua University
Current Affiliation: National Engineering Research Center for Beijing Biochip Technology, Capitalbio Corporation
Research Area: Biomedical engineering, microfluidic, nucleotide analysis, electrophoresis, biochemistry
Publications:
[1] Gan, W.P.,# Zhuang, B.,# Zhang, P.F., Han, J.P., Li, C.X., and Liu, P. (2014). A filter paper-based microdevice for low-cost, rapid, and automated DNA extraction and amplification from diverse sample types. Lab on a Chip 14, 3719-3728.
[2] Zhuang, B., # Gan, W.P., Wang, # S.Q., Han, J.P., Xiang, G.X., Li, C.X., Sun, J., Liu, P. (2015). A fully automated sample preparation microsystem for genetic testing of hereditary hearing loss using two-color multiplex allele-specific PCR. Analytical Chemistry, 87(2):1202-1209.
[3] Zhuang, B., # Han, J.P., # Xiang, G.X., Gan, W.P., Wang, S.Q., Wang, D., Wang, L., Sun, J., Li, C.X., Liu, P. (2016). A fully integrated and automated microsystem for rapid pharmacogenetic typing of multiple warfarin-related single-nucleotide polymorphisms. Lab on a Chip 16, 86-95.
"About the title" may belong to another edition of this title.
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