Cell Separation : Fundamentals, Analytical and Preparative Methods

Ashok Kumar

ISBN 10: 3540752625 / ISBN 13: 9783540752622

Published by Springer Berlin Heidelberg, 2007

About this Item

Druck auf Anfrage Neuware - Printed after ordering - Cell separation, which was once limited to merely being a basic technique for fractionating different cell populations, has come a long way in the last two decades. New, advanced and more speci c and selective techniques have emerged as the demand for isolating a speci c cell type for various biological applications has increased. Ef cient and cost-effective techniques for fr- tionation and isolation of target cell types are necessary to provide pure cell populations for diagnostics, biotechnological and biomedical applications. One can see a considerable need, both in biomedical research and in di- nostic medicine, for the speci c separation of a discrete population of cells from a mixture. For example, in the eld of tissue engineering, isolation of stemcellsfromtissuesororgansisofparticularlygreatimportance.Moreover, understanding cell developmental pathways becomes increasingly signi cant as diagnosis and treatment of diseases turns more to the molecular level. The diagnosis of cell-related diseases requires methods of detection, isolation and theanalysisofindividualcells,regardlessoftheirrelativecontentinthetissue. Since cell-based therapies now turn towards more realistic medical options, developing an effective separation system for large-scale cell separation has becomechallengingresearchgoalforcellbiologistsandbiotechnologists.The ideal technique should provide in a short time a good yield of cells with high puritywhile maintaining cellfunction.Despite the growingneed formethods to separate cells into cell subpopulations, the existing cell-separation te- niques stillhave somelimitations when the desired degree ofperformance on apreparativescaleisrequired.Wewillseemoreresearchfocusinthisdirection in the future. The traditional techniques of micro ltration, ultra ltration and ultrac- trifugation, which exploit differences in cell size, shape and density, have remainedtheworkhorsesdespitelowspeci cityandproblemswithscalingup.

Seller Inventory # 9783540752622

Bibliographic Details

• Title: Cell Separation : Fundamentals, Analytical and Preparative Methods
• Publisher: Springer Berlin Heidelberg
• Publication Date: 2007
• Language: English
• ISBN 10: 3540752625
• ISBN 13: 9783540752622
• Binding: Buch
• Condition: Neu
• Dimensions: 241W x 160H x 18D millimeters 241 millimeters width by 160 millimeters height by 18 millimeters depth
• Weight: 500 grams

About this title

Synopsis

Cell separation, which was once limited to merely being a basic technique for fractionating different cell populations, has come a long way in the last two decades. New, advanced and more speci?c and selective techniques have emerged as the demand for isolating a speci?c cell type for various biological applications has increased. Ef?cient and cost-effective techniques for fr- tionation and isolation of target cell types are necessary to provide pure cell populations for diagnostics, biotechnological and biomedical applications. One can see a considerable need, both in biomedical research and in di- nostic medicine, for the speci?c separation of a discrete population of cells from a mixture. For example, in the ?eld of tissue engineering, isolation of stemcellsfromtissuesororgansisofparticularlygreatimportance.Moreover, understanding cell developmental pathways becomes increasingly signi?cant as diagnosis and treatment of diseases turns more to the molecular level. The diagnosis of cell-related diseases requires methods of detection, isolation and theanalysisofindividualcells,regardlessoftheirrelativecontentinthetissue. Since cell-based therapies now turn towards more realistic medical options, developing an effective separation system for large-scale cell separation has becomechallengingresearchgoalforcellbiologistsandbiotechnologists.The ideal technique should provide in a short time a good yield of cells with high puritywhile maintaining cellfunction.Despite the growingneed formethods to separate cells into cell subpopulations, the existing cell-separation te- niques stillhave somelimitations when the desired degree ofperformance on apreparativescaleisrequired.Wewillseemoreresearchfocusinthisdirection in the future. The traditional techniques of micro?ltration, ultra?ltration and ultrac- trifugation, which exploit differences in cell size, shape and density, have remainedtheworkhorsesdespitelowspeci?cityandproblemswithscalingup.