Title: Cancer Cell Culture: Methods and Protocols (Methods in Molecular Medicine)
Publisher: Humana Press
Publication Date: 2003
Language: English
ISBN 10: 1588290794
ISBN 13: 9781588290793
Binding: Hardcover
Condition: Very Good
Book Type: book

About this title

Synopsis

Expert researchers describe in detail their most productive and up-to-date methods for growing cancer cells in the laboratory. There are methods to characterize and authenticate cell lines, to isolate and develop specific types of cancer cells, and to develop new cell line models. Functional assays are provided to evaluate clonogenicity, cell proliferation, apoptosis, adhesion, migration, invasion, senescence, angiogenesis, and cell cycle parameters. Other methods permit the modification of cancer cells for transfection, development of drug resistance, immortalization, and transfer in vivo; the co-culture of different cell types; and the detection and treatment of contamination.

Review

...I recommend this book as a good starting point to anyone new to cancer cell culture. There are many general principles to be gleaned from the authors' experience...if one gets frustrated after repeated unsuccessful attempts to establish, characterize, and manipulate one's own primary cancer cell line, Dr. Langdon has made sure there is an appendix that catalogues well-established cell lines, and provides the contacts necessary to obtain these cell lines. -- Cancer Biology & Therapy "...concise and clearly presented...interesting and straightforward information..." - Zellbiolgie Aktuell "Comprehensive and highly practical...provides both basic scientists and clinical researchers with a gold-standard collection of advanced techniques for culturing cancer cells effectively in their laboratories." - Tumori

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