CRISPR Guide RNA Design: Methods and Protocols

Fulga, Tudor A. (Edited by)/ Knapp, David J. H. F. (Edited by)/ Ferry, Quentin R. V. (Edited by)

ISBN 10: 1071606867 / ISBN 13: 9781071606865

Published by Humana, 2020

About this Item

1st edition. 286 pages. 10.00x7.00x0.75 inches. In Stock.

Seller Inventory # x-1071606867

Bibliographic Details

• Title: CRISPR Guide RNA Design: Methods and Protocols
• Publisher: Humana
• Publication Date: 2020
• Language: English
• ISBN 10: 1071606867
• ISBN 13: 9781071606865
• Binding: Hardcover
• Condition: Brand New

About this title

Synopsis

This detailed volume focuses on the CRISPR-associated guide RNA and how it can be designed, modified, and validated for a broad repertoire of purposes. Beginning with a section on computational design of target-specific guide RNAs, the book continues by covering chemical modifications to alter guide RNA stability, specificity, and efficiency, as well as to create inducible guide RNAs, append additional functional domains, and express guide RNAs in a conditional manner. It concludes with methods for measuring off-target guide RNA activity. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and essential, CRISPR Guide RNA Design: Methods and Protocols provides a comprehensive pipeline for guide RNA design and aims to be an invaluable resource in applying this powerful technology to basic research and therapeutic applications.