Biolistic DNA Delivery. Methods and Protocols
Language: English
Published by Humana Press, New York, 2013
- Hardcover
- Used
Seller: Research Ink, Takoma Park, MD, U.S.A.Research Ink
AbeBooks seller since August 1, 2000
Condition: Used - As new
£ 63.96
Quantity: 1 available
Add to basketItem description from seller
xiv + 415 pp. Methods in Molecular Biology, volume 940.
Seller Inventory # 27744
- Title
- Biolistic DNA Delivery. Methods and Protocols
- Author
- Sudowe, Stephan, and Angelika B. Reske-Kunz, Eds.
- Publisher
- Humana Press, New York
- Publication year
- 2013
- Condition
- As new
- Book Type
- book
- Binding
- Hardback
- Language
- English
- ISBN 10
- 162703109X
- ISBN 13
- 9781627031097
- Seller catalogs
- Biology
Biolistic transfection represents a direct physical gene transfer approach in which nucleic acids are precipitated on biologically inert high-density microparticles (usually gold or tungsten) and delivered directly through cell walls and/or membranes into the nucleus of target cells by high-velocity acceleration using a ballistic device such as the gene gun. Biolistic DNA Delivery: Methods and Protocols provides a comprehensive collection of detailed protocols intended to provide the definitive practical guide for the novice as well as for the advanced gene transfer expert on how to introduce nucleic acids into eukaryotic cells using the biolistic technique. Split into six convenient sections, this detailed volume covers biolistic gene transfer into plants, nematodes, and mammalian cells, both in vitro and in vivo, as well as the use of gene gun-mediated DNA vaccination in various experimental animal models of human diseases, and the description of biolistic delivery of molecules other than nucleic acids. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
All-inclusive and cutting-edge, Biolistic DNA Delivery: Methods and Protocols brings together the knowledge and the experience of leading experts in the field of gene transfer in order to serve all researchers who wish to further our abilities in this vital field.
"Synopsis" may belong to another edition of this title.
From the Back Cover
Biolistic transfection represents a direct physical gene transfer approach in which nucleic acids are precipitated on biologically inert high-density microparticles (usually gold or tungsten) and delivered directly through cell walls and/or membranes into the nucleus of target cells by high-velocity acceleration using a ballistic device such as the gene gun. Biolistic DNA Delivery: Methods and Protocols provides a comprehensive collection of detailed protocols intended to provide the definitive practical guide for the novice as well as for the advanced gene transfer expert on how to introduce nucleic acids into eukaryotic cells using the biolistic technique. Split into six convenient sections, this detailed volume covers biolistic gene transfer into plants, nematodes, and mammalian cells, both in vitro and in vivo, as well as the use of gene gun-mediated DNA vaccination in various experimental animal models of human diseases, and the description of biolistic delivery of molecules other than nucleic acids. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
All-inclusive and cutting-edge, Biolistic DNA Delivery: Methods and Protocols brings together the knowledge and the experience of leading experts in the field of gene transfer in order to serve all researchers who wish to further our abilities in this vital field."About the title" may belong to another edition of this title.
Research Ink
Takoma Park, MD, U.S.A.
AbeBooks seller since August 1, 2000
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