Bio-analytical method development of antimalarial drug in human plasma : Validation for quantitative estimation by using LC-MSMS

Language: English

Published by LAP LAMBERT Academic Publishing, 2019

6200482470 / 9786200482471

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Seller: AHA-BUCH GmbH, Einbeck, GermanyAHA-BUCH GmbH

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nach der Bestellung gedruckt Neuware - Printed after ordering - In the present work new, simple reversed-phase LCMS/MS in positive ion mode method was developed and validated for the determination of an anti-malarial in blood plasma. Drug was used as an internal standard. The chromatographic separation was achieved with Zorbax SB-C18, 5.0µm (150\*4.6mm). The separation of Drug H was achieved by gradient elution using organic mixture (Acetonitrile: Methanol::80:20 v/v), buffer solution (10mM Ammonium Formate) and DMSO:: 50:50:0.2 v/v/v. The flow rate is 1.000 ml/min, Injection Volume of 10µl with a run time of 3.0 minutes. The calibration curve of standard Drug H was linear in range 0.1-20.0 ¿g/ml. 5 precision and accuracy batches were run; the coefficient of correlation (r2) was ¿ 0.99. The accuracy of the LLOQ in the standard CC was within ± 20% of the nominal concentration and ± 15% of the nominal concentration for other standards and 75% non- zero calibration standards met the acceptable criteria which indicated the method was precise and accurate. Method validation results have proved the method to be selective, sensitive, precise, accurate and robustness.…

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Title
Bio-analytical method development of antimalarial drug in human plasma : Validation for quantitative estimation by using LC-MSMS
Author
Bincy Abraham
Publisher
LAP LAMBERT Academic Publishing
Publication year
2019
Condition
Neu
Binding
Taschenbuch
Language
English
ISBN 10
6200482470
ISBN 13
9786200482471
Item weight
209 grams
Dimensions
220x150x9 mm

AHA-BUCH GmbH

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