Bacterial Secretion Systems : Methods and Protocols

Eric Cascales

ISBN 10: 107163447X / ISBN 13: 9781071634479

Published by Springer US, Springer US, 2024

About this Item

Druck auf Anfrage Neuware - Printed after ordering - This second edition details new and updated protocols that cover techniques used to study secretion systems. Chapters focus on identifying and localizingthe different subunits, defining interactions within subunits, monitoring conformational changes, purifying and imaging of large complexes, defining the assembly pathway by fluorescence microscopy and the role of energy during assembly and/or secretion, identifying secreted effectors as well as using reporters to follow effector transport.Written in the highly successfulMethods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge,Bacterial Secretion Systems: Methods and Protocols- Second Editionaimsto be auseful andpractical guide to new researchersand experts looking to expand their knowledge.

Seller Inventory # 9781071634479

Bibliographic Details

• Title: Bacterial Secretion Systems : Methods and Protocols
• Publisher: Springer US, Springer US
• Publication Date: 2024
• Language: English
• ISBN 10: 107163447X
• ISBN 13: 9781071634479
• Binding: Taschenbuch
• Edition: 2nd Edition
• Condition: Neu

About this title

Synopsis

This second edition details new and updated protocols that cover techniques used to study secretion systems. Chapters focus on identifying and localizing the different subunits, defining interactions within subunits, monitoring conformational changes, purifying and imaging of large complexes, defining the assembly pathway by fluorescence microscopy and the role of energy during assembly and/or secretion, identifying secreted effectors as well as using reporters to follow effector transport. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Bacterial Secretion Systems: Methods and Protocols- Second Edition aims to be a useful andpractical guide to new researchers and experts looking to expand their knowledge.