Application of Microarray technology

Language: English

Published by LAP LAMBERT Academic Publishing Mai 2010, 2010

3838364066 / 9783838364063

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This item is printed on demand - Print on Demand Titel. Neuware -The construction of cDNA libraries is useful in understanding gene expression in organisms under different conditions, but random sequencing of unbiased cDNAs is laborious and gives rise to redundancy. We aimed to isolate cDNAs induced by switching Aspergillus nidulans from glucose to selected polysaccharides. We devised a Negative Subtraction Hybridization method which involved screening a library made from cDNAs from cells grown under a selected physiological condition with labeled cDNA probes from another physiological condition. In a screen for transcripts induced by switching the carbon source, 3,532 negatives were isolated. Single-channel microarray experiments were done to confirm that the majority of the negatives were indeed induced differentially. To gain insight into the regulation, we used microarrays to identify gene expression changes following a shift from glucose to pectin or glucose starvation. The findings from this study portray the useful utilization of the powerful microarray technology to study gene expression profiling under various physiological conditions.VDM Verlag, Dudweiler Landstraße 99, 66123 Saarbrücken 68 pp. Englisch.…

Seller Inventory # 9783838364063

Title
Application of Microarray technology
Author
Anamika Ray
Publisher
LAP LAMBERT Academic Publishing Mai 2010
Publication year
2010
Condition
Neu
Binding
Taschenbuch
Language
English
ISBN 10
3838364066
ISBN 13
9783838364063
Item weight
119 grams
Dimensions
220x150x5 mm

buchversandmimpf2000

Emtmannsberg, BAYE, Germany

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