This text presents the proceedings of the ninth symposium on Analytical Ultracentrifugation which was held at the Max Delbruck Center for Molecular Medicine in Berlin-Buch during March 1995. It presents a survey of recent developments and results of ultracentrifugation methods in the frame of the following topics: reversible association reactions; analysis of supramo. It presents a survey of recent developments and results of ultracentrifugation methods in the frame of the following topics: reversible association reactions; analysis of supramolecular structures including modelling; size distribution analysis and gel formation; and hardware and software developments.
From the reviews a ]
"This is a hefty book with a hefty price tag and a hefty number of mathematical equations throughout. So why would a novice coming into this field with a biological background buy it? Well for starters, it would be useful for any group or department that has an analytical ultracentrifuge (AUC), or has an interest in one.
Those people with little knowledge of mathematics need not fear. The authors have done an excellent job in explaining what the equations mean and their relevance. On top of this they have translated them into nice little computational algorithms and written programmes which handle all the calculations at the click of a mouse button. So simple that even a novice can handle the data analysis.
So what does an analytical ultracentrifuge do? A quick perusal of the book and I see that the machine is capable of tracking the sedimentation of macromolecules in a solvent. So what?
The AUC is used to study proteins, DNA, polysaccharides, membrane proteins, polymers, colloids and nanoparticles. The AUC can tell you if the molecules in solution are homogeneous, multimeric or have multi components. You can calculate the molecular weight, the distribution of molecular species and the affinities between them. You can estimate the rough size and shape of molecules. All this and you can even get your molecules back. This book is essential for planning a sedimentation velocity or equilibrium experiment. The chapters are sensibly laid out with a general introduction to centrifugation, description of the thermodynamic behaviour of molecules in solution, and description of software packages for data analysis. It is brimming with very useful advice. A must for all biochemists and biophysicists."
Jayesh Gor, CHEMISTRY WORLD, Vol. 3, No. 7, July 2006.
From the reviews
"This is a hefty book with a hefty price tag and a hefty number of mathematical equations throughout. So why would a novice coming into this field with a biological background buy it? Well for starters, it would be useful for any group or department that has an analytical ultracentrifuge (AUC), or has an interest in one.
Those people with little knowledge of mathematics need not fear. The authors have done an excellent job in explaining what the equations mean and their relevance. On top of this they have translated them into nice little computational algorithms and written programmes which handle all the calculations at the click of a mouse button. So simple that even a novice can handle the data analysis.
So what does an analytical ultracentrifuge do? A quick perusal of the book and I see that the machine is capable of tracking the sedimentation of macromolecules in a solvent. So what?
The AUC is used to study proteins, DNA, polysaccharides
A valuable reference for anyone involved or interested in all aspects of modern AUC with a good perspective of the "state-of-the-art." The recent acceleration in AUC technology, efficiently documented in this book.....well executed book.
