Acidic Protease Production in Air Lift Bioreactor by Rhizopus Arrhizus

Language: English

Published by LAP Lambert Academic Publishing Jul 2016, 2016

3846502545 / 9783846502549

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Seller: BuchWeltWeit Ludwig Meier e.K., Bergisch Gladbach, GermanyBuchWeltWeit Ludwig Meier e.K.

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This item is printed on demand - it takes 3-4 days longer - Neuware -The project was planned to produce max. protease from Whey by Rhizopus arrhizus at pH 4 and 37C temperature. Growth media employed to culture R. arrhizus for the production of enzyme were developed and fermentation conditions was optimized through various trials. Substrate water ratio, different nitrogen sources and cont. of nitrogen source were optimized. The fermented materials were harvested after 72 hours. These were filtered and centrifuged at 10,000 rpm at -10C. The filtrates were subjected to enzyme assay. Absorbance of the enzyme sample was determined at 660 nm on spectrophotometer. The enzyme activity was calculated and the results were analyzed statistically by using ANOVA under CRD and by taking MSE from ANOVA, DMR test w sap-lied to compare the treatment means. It was observed that 90% Whey and 2.5% cotton seed meal enhanced the production of protease by R. arrhizus. Max. enzyme activity was observed (149.26 IU/ml/min) in flask level at pH 4 and 37C temperature. These optimized conditions of growth media was again used in Air-Lift fermenter and determined the activity (169.78 IU/ml/min) that is greater than flask level. This is due to temperature in Air-lift fermenter. 64 pp. Englisch.

Seller Inventory # 9783846502549

Title
Acidic Protease Production in Air Lift Bioreactor by Rhizopus Arrhizus
Author
Azeem Haider
Publisher
LAP Lambert Academic Publishing Jul 2016
Publication year
2016
Condition
Neu
Binding
Taschenbuch
Language
English
ISBN 10
3846502545
ISBN 13
9783846502549
Item weight
113 grams
Dimensions
220x150x5 mm

BuchWeltWeit Ludwig Meier e.K.

Bergisch Gladbach, Germany

5-star seller

AbeBooks seller since January 11, 2012

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BuchWeltWeit Ludwig Meier e.K.

Germany