A Physicochemical Perspective On Evolutionarily Related Repeated, Interspaced, Palindromic Sequence-Associated Effectors For Editing Genetic Material - Softcover
Synopsis
The CRISPR/Cas9 system is a naturally occurring, adaptive heritable immune system which defends invading phage attack and present in the whooping numbers of prokaryotes.This has been repurposed as a powerful tool which is guided by RNA and targets DNA for genome engineering which works by making site-specific DNA break and its subsequent repair by cellular machinery. The simplicity and multiplexity of the CRISPR system make it more sought after than other older counterparts viz. ZFNs and TALENs, and this has led to harnessing of CRISPR in gene therapy programmable gene regulation, cellular imagings, synthetic biology and nucleic acid detection among others. The CRISPR genome editing system includes an endonuclease, Cas9 derived from type II CRISPR-Cas system and a chimeric single-guide RNA (sgRNA). Cas9 and sgRNA forms ribonucleoprotein (RNP) binary complex and upon recognition of a protospacer adjacent motif (PAM) it binds to the substrate DNA, and forms ternary complex by unwinding the PAM upstream region with subsequent formation of R-loop. Successful R-loop formation displaces PAM- containing DNA strand and results in formation of a heteroduplex only when the target DNA strand finds complementary to sgRNA sequence. It is worth mentioning Cas9 remains inactive inthe absence of sgRNA and sgRNA binding induces structural changes in Cas9 from apo toactive form.
"synopsis" may belong to another edition of this title.
Search results for A Physicochemical Perspective On Evolutionarily Related...
Stock Image
A Physicochemical Perspective On Evolutionarily Related Repeated, Interspaced, Palindromic Sequence-Associated Effectors For Editing Genetic Material
Print on DemandSeller: PBShop.store US, Wood Dale, IL, U.S.A.
Seller rating 5 out of 5 stars
PAP. Condition: New. New Book. Shipped from UK. THIS BOOK IS PRINTED ON DEMAND. Established seller since 2000. Seller Inventory # L0-9798230131236
Stock Image
A Physicochemical Perspective On Evolutionarily Related Repeated, Interspaced, Palindromic Sequence-Associated Effectors For Editing Genetic Material
Print on DemandSeller: PBShop.store UK, Fairford, GLOS, United Kingdom
Seller rating 5 out of 5 stars
PAP. Condition: New. New Book. Delivered from our UK warehouse in 4 to 14 business days. THIS BOOK IS PRINTED ON DEMAND. Established seller since 2000. Seller Inventory # L0-9798230131236
Buy New
£ 27.67
£ 4.16 shipping
Ships from United Kingdom to U.S.A.
Ships from United Kingdom to U.S.A.
Quantity: Over 20 available
Stock Image
A Physicochemical Perspective On Evolutionarily Related Repeated, Interspaced, Palindromic Sequence-Associated Effectors For Editing Genetic Material
Seller: Ria Christie Collections, Uxbridge, United Kingdom
Seller rating 5 out of 5 stars
Condition: New. In. Seller Inventory # ria9798230131236_new
Buy New
£ 27.57
£ 11.98 shipping
Ships from United Kingdom to U.S.A.
Ships from United Kingdom to U.S.A.
Quantity: Over 20 available
Seller Image
A Physicochemical Perspective On Evolutionarily Related Repeated, Interspaced, Palindromic Sequence-Associated Effectors For Editing Genetic Material
Seller: AHA-BUCH GmbH, Einbeck, Germany
Seller rating 5 out of 5 stars
Taschenbuch. Condition: Neu. Neuware - The CRISPR/Cas9 system is a naturally occurring, adaptive heritable immune system which defends invading phage attack and present in the whooping numbers of prokaryotes.This has been repurposed as a powerful tool which is guided by RNA and targets DNA for genome engineering which works by making site-specific DNA break and its subsequent repair by cellular machinery. The simplicity and multiplexity of the CRISPR system make it more sought after than other older counterparts viz. ZFNs and TALENs, and this has led to harnessing of CRISPR in gene therapy programmable gene regulation, cellular imagings, synthetic biology and nucleic acid detection among others. The CRISPR genome editing system includes an endonuclease, Cas9 derived from type II CRISPR-Cas system and a chimeric single-guide RNA (sgRNA). Cas9 and sgRNA forms ribonucleoprotein (RNP) binary complex and upon recognition of a protospacer adjacent motif (PAM) it binds to the substrate DNA, and forms ternary complex by unwinding the PAM upstream region with subsequent formation of R-loop. Successful R-loop formation displaces PAM- containing DNA strand and results in formation of a heteroduplex only when the target DNA strand finds complementary to sgRNA sequence. It is worth mentioning Cas9 remains inactive inthe absence of sgRNA and sgRNA binding induces structural changes in Cas9 from apo toactive form. Seller Inventory # 9798230131236