HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY AND MASS SPECTROMETRY OF PORPHYRINS, CHLOROPHYLLS AND BILINS: 2 (Methods In Chromatography) - Hardcover

LIM CHANG-KEE

Hardcover

ISBN 10: 9810230680 ISBN 13: 9789810230685

Publisher: World Scientific Publishing, 2009

Synopsis

The porphyrins, chlorophylls, bilins and related tetrapyrroles are vital for all living organisms. Natural and synthetic tetrapyrroles are used extensively in foods, cosmetics, biotechnology, pharmaceuticals, diagnostics and medicine. Methods for their separation and characterization therefore, have a very wide area of applications. Yet, there is a dearth of books dedicated to HPLC and HPLC/MS of tetrapyrroles. Lim addresses this problem admirably by providing practical HPLC and HPLC/MS protocols coupled with in-depth chromatographic and mass spectrometric reference data. These are invaluable in the analysis, identification and characterization of porphyrins, chlorophylls, bilins and other related compounds found in biological and clinical materials. HPLC method development and optimization for coupling to mass spectrometry are also described in rich detail. Sample preparation, and suggestions for avoiding procedural artifacts during extraction of clinical and biological samples are discussed. Clinical biochemists involved in biochemical diagnosis of human porphyrias will find this monograph assuredly helpful, as would analysts, biochemists and chemists involved in the separation, isolation and characterization of natural and synthetic tetrapyrroles. Undoubtedly, Lim has contributed a master-piece containing sufficient background material for beginners and up-to-date references for all researchers in the field.

About this title

Synopsis

Presents a compilation of HPLC methods for the separation and quantitation of porphyrins and porphyrin precursors. The physical-chemical background, structure-retention relationships and optimization of separation of porphyrins and porphyrinogens by reversed-phase HPLC are discussed. There are practical tips based on application-oriented examples, including detailed instructions and protocols, from sample collection, handling and storage preparation, and HPLC separation and quantitation. Methods for measurement of haem biosynthetic enzyme activities are described, and characteristic porphyrin profiles in the blood, urine and faeces of patients with different types of porphyrias are given. There are also chapters relating to TLC and capillary electrophoresis (CE) of porphyrins.

Edition details

• Publisher: World Scientific Publishing
• Publication date: 2009
• Language: English
• ISBN 10: 9810230680
• ISBN 13: 9789810230685
• Binding: Hardcover
• Number of pages: 244