Methods in Biotechnology and Bioengineering is a self-contained compilation of laboratory experimental protocols to meet the need of students, experienced researchers and newcomers to the biotechnology discipline with diversified biotechnological interests. It provides highly useful experimental details on immobilization technology, cell and tissue culture, electrophoresis, conjugation techniques, fermentation, enzyme isolation and characterization, DNAIRNA isolation, cloning and analysis, hybridoma technology, immunological techniques (covering immunization and immunodiagnostics). The protocols are contained in different relevant chapters. Every chapter starts with concise and most relevant introduction to the chapter followed by algorithm of various experimental methods. Methods are very well developed and tested in laboratories from all over the world and presented in this volume with adaptable modifications to make them easily workable. Emphasis has been placed on qualitative procedure, central strategy and alternatives to the methods. Also at the end, index for at-a-glance accessibility is provided. Hope this book will be a highly helpful instruction guide for self-training of the students in the laboratory without requiring any instructive and demonstrative helps.1 Cell Culture Techniques 2 Electrophoresis. 3 Conjugation Techniques 4 Fermentation 5 Biochemical Preparations 6 Enzyme Linked Immunosorbent Assay (ELISA) 7 Enzyme and Cell Immobilization 8 DNA and RNA Isolation 9 Cloning and Molecular Analysis of Genes 10 Hybridoma Cell Technology 11 Immunological Methods.
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Prof. S. P.Vyas has nearly 22 years of research and teaching experience at both U.G. and P.G. levels. He has worked as Manager, R&D (New Drug Delivery) in Lupin Laboratories, Aurangabad for a period of nearly one year. He is a leading scientist and well-known academician of international repute. Prof. Vyas did his postdoctoral research at the School of Pharmacy, University of London (U.K.) under the supervision of Prof. Gregory Gregoriadis, a renowned liposomologist. He has supervised 17 PI and more than 55 M. Pharm. research projects. He has over 150 research publications to his credit published in international and national journals. He has been awarded Pt. Lajja Shankar Jha Award for excellence in research in Engineering and Technology by the Govt. of Madhya Pradesh. He is a recipient of Motan Devi Dandiya Oration Award, 1998 and recently he has been honoured with Best Pharmacy Teacher of the Year, 2001 Award by APTI. He has delivered invited lectures, keynote addresses and chaired many sessions in several National and International Conferences and Symposia in India and abroad. He has written many invited review articles in Advanced Drug Delivery Reviews, Critical Reviews, TM in Therapeutic Drug Carrier Systems, Pharmaceutical Research and International Journal of Medicine and written many chapters in national and international books. His research interest extends from Novel Drug Delivery Systems to Delivery Aspects of Biopharmaceuticals, Molecular Basis of Drug Targeting, Biopharmaceuticals, new Drug Delivery Systems with biotechnology bias and Molecular Immunology. He has authored 4 books, entitled Pharmaceutical Biotechnology, Advances in Liposomal Therapeutics, Controlled Drug Delivery and Targeted and Controlled Drug Delivery. Presently, he is Professor (Pharmaceutical Biotechnology) and Dean, Faculty of Technology, Dr. Harisingh Gour University, Sagar. Dr. D.V. Kohil has nearly twenty two years of teaching experience both at undergraduate and postgraduate levels. He has vast and variegated experience in teaching and research. He is a well renowned scientist who has published over forty research papers in journals of international and national repute. He has supervised nearly thirtyM.Pharm. and two Ph.D. For his quality research work he has been awarded the IDMA research award for the year 1982. He has participated in various national and international conferences as resource person. He is a life member of IPA, MPPGA and APTI. His special research interest is in biochemical, biotechnological and synthetic bioanalogues. Presently, he is Reader in the Department of Pharmaceutical Sciences, Dr. H.S. GourVishwavidyalaya, Sagar. He is involved in research activities and is the principal investigator of some minor and major research projects sponsored by UGC and AICTE.
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