1、概述了测序技术发展历史,包括第一代、第二代和第三代测序技术的简介,及其在农学和育种学、医学与疾病分子诊断中的应用。2、全面阐述了Illumina测序技术的文库构建,包括DNA、RNA、小RNA、DNA序列捕获、简化基因组测序以及单细胞测序等建库技术及文库质量检测;详细说明了Illumina测序仪器HiSeq、MiSeq、NextSeq500的测序操作流程;详细介绍了下机数据的处理和分析流程,并简要介绍了目前常用的测序数据分析软件工具。3、详细阐述了PacBioRS的测序过程,包括建库、测序仪运行和下机数据处理过程
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Seller: liu xing, Nanjing, JS, China
paperback. Condition: New. Paperback. Pub Date: 2016-06-01 Pages: 360 Language: Chinese Publisher: Science Press. a limited liability company. an overview of the historical development of sequencing technology. including the profile of the first generation. second generation and third generation sequencing technology. and its application in agronomy and breeding. molecular medicine and disease diagnosis. 2. a comprehensive exposition of the library Illumina sequencing technology to build. including DNA. RNA. small RNA. Seller Inventory # DM025491