Studies of blaOXA Class D Producing Bacteria in Kano State, Nigeria: Prevalence of Class D OXA Beta-lactamases in Clinical Bacterial Isolate in Kano State, Nigeria - Softcover

Shu'aibu, Samira Salisu; Arzai, Auwal Halliru

Softcover

ISBN 10: 6200323631 ISBN 13: 9786200323637

Publisher: LAP LAMBERT Academic Publishing, 2019

Synopsis

This study was carried out with the aim to defect the presence and prevalence of newly discovered class DOXA β-lactamase, phenotypically and genotyphically. To determine the antibiotic susceptibility profile as well as its distribution among the male and female patients. The isolates were gram – negative bacteria obtained from three different hospitals in kano metropolis there include Escherichia coli, Klebsiella pneumonia, Pseudomonas aureginos, Proteus spp and Salmonella sp. A total of 500 clinical isolates were screened phenotypically using double discs synergy and by molecular methods with specific primers of blaoxa, and blaoxa 48 type and the PCR products were sequenced analyzed. Antimicrobial sensitivity test were performed among the positive isolates according to the clinical and laboratory standard institutes guidelines (CLSI). The results obtained showed the prevalence of the enzyme with Escherichia coli (10%), Klebsiella pneumonia (68%) Pseudomonas aureginosa (2.8%), Proteus mirabilis (4.0%), P. vulgaris (1.4%), S. typhi (1.0%) and S. paratyphi (0.4%) among the 32 isolates that were screened for PCR 9 were blaoxa1 positive.

Edition details

• Publisher: LAP LAMBERT Academic Publishing
• Publication date: 2019
• Language: English
• ISBN 10: 6200323631
• ISBN 13: 9786200323637
• Binding: Paperback
• Number of pages: 136