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A study of ligand-binding properties of Hepatocyte Nuclear Factor 4α: A study of the ligand-binding properties of Hepatocyte Nuclear Factor 4α, using a DAUDA fluorescence displacement assay - Softcover
Synopsis
Hepatocyte nuclear factor 4α (HNF4α) is a liver enriched transcription factor that belongs to the nuclear receptor family, and controls the expression of genes involved in various different functions, such as nutrient transport, metabolism, growth and differentiation. Recently, fatty acids and/or fatty acyl CoAs as well as some xenobiotics (e.g. hypolipidemic drugs) have been suggested as HNF4α ligands. The aim of this study was to identify potential ligands for HNF4α, using a DAUDA fluorescence displacement assay. LFABP was expressed and purified as a positive control, and used successfully to validate the DAUDA assay. Rat HNF4αLBD was successfully expressed and purified, therefore further work for ligand identification was performed using the rat HNF4α. Suitability of the DAUDA fluorescence displacement assay for screening of ligands for rat HNF4α was confirmed by a characteristic blue shift in fluorescence emission upon protein binding, as previously reported. Ligands screened in this study included five different fatty acids, palmitoyl CoA, and hypolipidemic xenobiotics of the fibrate family (fenofibrate, bezafibrate, clofibrate), as well as pioglitazone (a thionolidinedione).
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About the Author
Maria Papachristodoulou (BSc Hons, MPhil,University of Surrey, UK) is a Biology teacher in Cyprus. Dr Alfred Thumser (PhD,University of Cape Town) is a Lecturer in Biochemistry at the University of Surrey. Dr Nick Plant (PhD, University of Nottingham, UK) is a Senior Lecturer in Molecular Toxicology at the University of Surrey.
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A study of ligand-binding properties of Hepatocyte Nuclear Factor 4a
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LAP LAMBERT Academic Publishing, 2012
ISBN 10: 3846580821
ISBN 13: 9783846580820
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Condition: New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. Autor/Autorin: Papachristodoulou MariaMaria Papachristodoulou (BSc Hons, MPhil,University of Surrey, UK) is a Biology teacher in Cyprus. Dr Alfred Thumser (PhD,University of Cape Town) is a Lecturer in Biochemistry at the University of Surrey. Dr N. Seller Inventory # 5501061
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A study of ligand-binding properties of Hepatocyte Nuclear Factor 4 : A study of the ligand-binding properties of Hepatocyte Nuclear Factor 4 , using a DAUDA fluorescence displacement assay
Published by
LAP Lambert Academic Publishing, 2012
ISBN 10: 3846580821
ISBN 13: 9783846580820
New
Taschenbuch
Seller: AHA-BUCH GmbH, Einbeck, Germany
Seller rating 5 out of 5 stars
Taschenbuch. Condition: Neu. nach der Bestellung gedruckt Neuware - Printed after ordering - Hepatocyte nuclear factor 4 (HNF4 ) is a liver enriched transcription factor that belongs to the nuclear receptor family, and controls the expression of genes involved in various different functions, such as nutrient transport, metabolism, growth and differentiation. Recently, fatty acids and/or fatty acyl CoAs as well as some xenobiotics (e.g. hypolipidemic drugs) have been suggested as HNF4 ligands. The aim of this study was to identify potential ligands for HNF4 , using a DAUDA fluorescence displacement assay. LFABP was expressed and purified as a positive control, and used successfully to validate the DAUDA assay. Rat HNF4 LBD was successfully expressed and purified, therefore further work for ligand identification was performed using the rat HNF4 . Suitability of the DAUDA fluorescence displacement assay for screening of ligands for rat HNF4 was confirmed by a characteristic blue shift in fluorescence emission upon protein binding, as previously reported. Ligands screened in this study included five different fatty acids, palmitoyl CoA, and hypolipidemic xenobiotics of the fibrate family (fenofibrate, bezafibrate, clofibrate), as well as pioglitazone (a thionolidinedione). Seller Inventory # 9783846580820
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A study of ligand-binding properties of Hepatocyte Nuclear Factor 4 | A study of the ligand-binding properties of Hepatocyte Nuclear Factor 4 , using a DAUDA fluorescence displacement assay
Published by
LAP Lambert Academic Publishing, 2012
ISBN 10: 3846580821
ISBN 13: 9783846580820
New
Taschenbuch
Seller: preigu, Osnabrück, Germany
Seller rating 5 out of 5 stars
Taschenbuch. Condition: Neu. A study of ligand-binding properties of Hepatocyte Nuclear Factor 4 | A study of the ligand-binding properties of Hepatocyte Nuclear Factor 4 , using a DAUDA fluorescence displacement assay | Maria Papachristodoulou (u. a.) | Taschenbuch | Englisch | LAP Lambert Academic Publishing | EAN 9783846580820 | Verantwortliche Person für die EU: preigu GmbH & Co. KG, Lengericher Landstr. 19, 49078 Osnabrück, mail[at]preigu[dot]de | Anbieter: preigu. Seller Inventory # 106621113