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Functional Analysis of Promoter of cus Operon: Activity of Copper-mediated Induction of cus Operon Promoter in E. coli. - Softcover
Copper is an essential trace metal for both prokaryotes and eukaryotes, and is associated with various metal enzymes which play many essential roles in the living system. But excess of the metal is very toxic and even lethal for living organisms. In microorganisms the intracellular level of copper is maintained by many genes working as an operon and induced by the presence of copper in their environment. One such operon is called cus operon regulated by cusRS two component system in Klebsiella pneumoniae. This cusS (sensory region) and cusR (regulatory region) autoregulate the expression of structural as well as regulatory part of the cus operon by binding of CusR protein to the bidirectional promoter part of the operon.The functional analysis showed that the cloned promoter was highly sensitive to copper metal concentrations and follows Michaelis Menton kinetics at very high concentrations. Additionally, cusRS promoter was also scanned for various transcriptional factor binding sites like -10, -35 boxes for binding of RNA polymerase. Similarly, CusR binding region was highlighted after docking of CusR protein on promoter sequence via Hex Dock software.
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Nageena Zahid,M.Phil. is a Lecturer in Institute of Biotechnology, Bahauddin Zakariya University, Multan. Abdul Rauf Shakoori is a Distinguished National Professor and Director, School of Biological Sciences, University of the Punjab. He is Fellow of Academy of Sciences for Developing World (TWAS) and Fellow of Pakistan Academy of Sciences.
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- PublisherLAP LAMBERT Academic Publishing
- Publication date2011
- ISBN 10 3846505110
- ISBN 13 9783846505113
- BindingPaperback
- Number of pages76
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Functional Analysis of Promoter of Cus Operon
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Functional Analysis of Promoter of cus Operon: Activity of Copper-mediated Induction of cus Operon Promoter in E. coli.
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Functional Analysis of Promoter of cus Operon Activity of Coppermediated Induction of cus Operon Promoter in E coli
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Book Description Taschenbuch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Copper is an essential trace metal for both prokaryotes and eukaryotes, and is associated with various metal enzymes which play many essential roles in the living system. But excess of the metal is very toxic and even lethal for living organisms. In microorganisms the intracellular level of copper is maintained by many genes working as an operon and induced by the presence of copper in their environment. One such operon is called cus operon regulated by cusRS two component system in Klebsiella pneumoniae. This cusS (sensory region) and cusR (regulatory region) autoregulate the expression of structural as well as regulatory part of the cus operon by binding of CusR protein to the bidirectional promoter part of the operon.The functional analysis showed that the cloned promoter was highly sensitive to copper metal concentrations and follows Michaelis Menton kinetics at very high concentrations. Additionally, cusRS promoter was also scanned for various transcriptional factor binding sites like -10, -35 boxes for binding of RNA polymerase. Similarly, CusR binding region was highlighted after docking of CusR protein on promoter sequence via Hex Dock software. 76 pp. Englisch. Seller Inventory # 9783846505113
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Functional Analysis of Promoter of cus Operon Activity of Coppermediated Induction of cus Operon Promoter in E coli
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Functional Analysis of Promoter of cus Operon : Activity of Copper-mediated Induction of cus Operon Promoter in E. coli.
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Book Description Taschenbuch. Condition: Neu. nach der Bestellung gedruckt Neuware - Printed after ordering - Copper is an essential trace metal for both prokaryotes and eukaryotes, and is associated with various metal enzymes which play many essential roles in the living system. But excess of the metal is very toxic and even lethal for living organisms. In microorganisms the intracellular level of copper is maintained by many genes working as an operon and induced by the presence of copper in their environment. One such operon is called cus operon regulated by cusRS two component system in Klebsiella pneumoniae. This cusS (sensory region) and cusR (regulatory region) autoregulate the expression of structural as well as regulatory part of the cus operon by binding of CusR protein to the bidirectional promoter part of the operon.The functional analysis showed that the cloned promoter was highly sensitive to copper metal concentrations and follows Michaelis Menton kinetics at very high concentrations. Additionally, cusRS promoter was also scanned for various transcriptional factor binding sites like -10, -35 boxes for binding of RNA polymerase. Similarly, CusR binding region was highlighted after docking of CusR protein on promoter sequence via Hex Dock software. Seller Inventory # 9783846505113
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Functional Analysis of Promoter of cus Operon
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Book Description Condition: New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. Autor/Autorin: Zahid NageenaNageena Zahid,M.Phil. is a Lecturer in Institute of Biotechnology, Bahauddin Zakariya University, Multan. Abdul Rauf Shakoori is a Distinguished National Professor and Director, School of Biological Sciences, University . Seller Inventory # 5495241
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