Peste des Petits Ruminants Virus (PPRV): Comparative Analysis of Antigen Detection Methods - Softcover

Abubakar, Muhammad; Ashiq, Samina; Hussain, Zulkifal

Softcover

ISBN 10: 3845440805 ISBN 13: 9783845440804

Publisher: LAP LAMBERT Academic Publishing, 2011

Synopsis

Peste des petits ruminants (PPR) is an acute and highly contagious viral disease of small ruminants such as goats and sheep. In Pakistan it causes economic losses of Rs. 20.5 billion annually. The diagnosis of PPR infection in sheep and goats populations can be synergistically strengthened with detection of antigen in clinical samples of susceptible population. In present study the PPR virus antigen detection was investigated and compared in clinical samples of sheep and goats by Haemagglutination (HA) test, Agar gel immunodiffusion (AGID) and Immunocapture ELISA (IC-ELISA). The viral antigen was detected from 8.95% of the samples tested by AGID, 20.9% tested by HA test and 31.25% tested by IC-ELISA. Kappa statistics scores for HA versus AGID, IC-ELISA versus AGID and HA versus IC-ELISA were 0.45 (95% Confidence Interval CI 0.158-0.742), 0.359 (95% CI 0.01-0.69) and 0.035 (95% CI -0.309-0.694) respectively which indicate a non-significant agreement between the tests. The results of chi-square for the three tests (HA, AGID and IC-ELISA) indicated these tests are highly significance i.e. locations and tests results are associated with each other.

About this title

About the Author

Dr. Muhammad Abubakar, obtained his master’s degree in Veterinary Medicine in 2003. Since then he is exclusively involved in dealing Transboundary Animal Diseases (TADs) at National and International levels. He is the author of several articles on issues of livestock diseases in well reputed jourals and involved in various projects working on TADs.

Edition details

• Publisher: LAP LAMBERT Academic Publishing
• Publication date: 2011
• Language: English
• ISBN 10: 3845440805
• ISBN 13: 9783845440804
• Binding: Paperback
• Number of pages: 72