Development of a new inactivated virus vaccine: Case study for Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) - Softcover

Delrue, Iris

Softcover

ISBN 10: 3845402822 ISBN 13: 9783845402826

Publisher: LAP LAMBERT Academic Publishing, 2011

Synopsis

This book outlines the different stages of vaccine development and focuses on molecular methods that can be used for screening and quality control for inactivated virus vaccines. First, a screening assay was developed to evaluate virus inactivation and preservation of the virion structure. Second, possible vaccine virus adaptation problems to a cell line are solved. This research is based on porcine reproductive and respiratory syndrome virus (PRRSV), but can be valuable for development of other vaccines as well.PRRSV leads to reproduction failure in sows and is associated with the respiratory disease complex in pigs. Although commercial vaccines exist, PRRSV is still difficult to control in the field. This can be due to modification of epitopes during the inactivation procedure or virus adaptation to a cell line.We developed a new inactivated PRRSV vaccine with virus grown on a PRRSV sensitive pig cell line to minimize mutations in viral structural proteins. Furthermore, we screened different inactivation methods for their ability to preserve viral entry-associated domains. These measures enabled us to select in vitro for the vaccine that afterwards showed best results in vivo.

About this title

About the Author

Iris Delrue, PhD in Veterinary Sciences (vaccine development), M.Sc. Biotechnology at Ghent University, Belgium.

Edition details

• Publisher: LAP LAMBERT Academic Publishing
• Publication date: 2011
• Language: English
• ISBN 10: 3845402822
• ISBN 13: 9783845402826
• Binding: Paperback
• Number of pages: 172