CHARACTERIZE MAMMALIAN TRANSCRIPTOME COMPLEXITY: FROM GENOME-WIDE RT-PCR CDNA CLONING, TO NOVEL MAMMALIAN GENE DISCOVERY, AND TRANSCRIPTOME COMPLEXITY - Softcover

Wu, Jiaqian

Softcover

ISBN 10: 3844320601 ISBN 13: 9783844320602

Publisher: LAP LAMBERT Academic Publishing, 2011

Synopsis

In my Ph.D. thesis, I described a large scale RT-PCR and cloning pipeline that I developed to obtain several thousand full-length mammalian (mainly human and mouse) cDNA clones for the NHGRI/NCI Mammalian Gene Collection effort. At that time, the layers of the mammalian transcriptome complexity were not yet fully conceived. In this project, I was deeply involved in strategic and conceptual issues in characterizing transcriptome complexity from different levels. Using the RT-PCR pipeline combined with gene prediction algorithms that utilize comparative genomic information, we successfully identified and experimentally verified novel mammalian genes such as full-length rat homologs of human disease genes. Furthermore, I studied transcriptome complexity including SNPs and alternative splicing in leukemia samples using quantitative PCR, and we developed a trace analysis technology to identify multiple alternative spliced transcripts in one single sequencing reaction. My results suggested that alternatively spliced transcripts show cancer type specific up- or down- regulation, and the ratio of alternative spliced isoforms may underlie the cancer mechanism.

About this title

About the Author

Jiaqian Wu, Ph.D. authored many peer-reviewed publications in the field of Genetics, Genomics and Stem Cell research. She won multiple awards and has one patent. Jiaqian has given talks at international conferences and research institutes. Jiaqian is currently an assistant professor at the University of Texas Medical School at Houston.

Edition details

• Publisher: LAP LAMBERT Academic Publishing
• Publication date: 2011
• Language: English
• ISBN 10: 3844320601
• ISBN 13: 9783844320602
• Binding: Paperback
• Number of pages: 204