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Parallel Single Cell Electrophoresis in a Microchip Platform - Softcover

Munce, Nigel; Lilge, Lothar

 
9783838304489: Parallel Single Cell Electrophoresis in a Microchip Platform

Synopsis

Single cell analysis has typically been difficult and challenging to perform in such a way that meaningful statistical information can be extracted. This thesis documents the development of a microchip or lab-on-a-chip platform to perform single cell electrophoresis in parallel separation channels. It describes the use of optical tweezers to select a cell and transport it to a desired separation channel. It also describes the use of electromechanical shearing to lyse a cell. These two techniques are combined with laser-micromachined separation channels to perform simple electrohoresis on the fluorescently labeled cellular contents.

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About the Author

Nigel Munce is currently a research scientist and medical student at the University of Toronto in Canada. He is interested in both medical devices and the development of new medical imaging techniques.

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