BIOLOGICAL CELL MEMBRANE DYNAMICS INDUCED BY ELECTRIC PULSES- Background, Experiment and Modeling - Softcover

Ji, Zhen

Softcover

ISBN 10: 3836455366 ISBN 13: 9783836455367

Publisher: VDM Verlag Dr. Mueller E.K., 2007

Synopsis

Electroporation has been found to be a very effective approach to introduce low and high molecular weight molecules such as drugs, hormones, proteins, DNA, and chromosomes into biological cells. Although electroporation has been under investigation for long time, the dynamic formation and resealing of pores is still not completely understood. In this work, we first computationally analyzed the electric field dosimetry for a Gigahertz Trans­verse Electromagnetic chamber. We found that this exposure method suffered from undesirable field polarity oscillations. Using a novel micro-fabricated microcuvette and pulse delivering system, we conducted a series of experimental studies of electroporation of HL-60 cells. Theoretical models were also employed to describe the mechanisms for pore formation and development of a spherical (HL-60) cell. A locally constrained surface tension model was proposed to represent the cytoskeleton and anchor proteins in the cell membrane and was observed to provide a plausible explanation for experi­mental evidence of long-lived pores. Also, transport mechanisms of small charged molecules associated with electroporation were analyzed.

About this title

About the Author

was born in Dalian, China on the 29thof December 1970. He received a BS fromTsinghua University in 1994, a MS from Rose-Hulman Institute of Technology in 2003 and a PhDfrom the University of Wisconsin-Madison in 2007.His major research area is BioElectromagnetics,employing both theoretical and experimentalmethods.

Edition details

• Publisher: VDM Verlag Dr. Mueller E.K.
• Publication date: 2007
• Language: English
• ISBN 10: 3836455366
• ISBN 13: 9783836455367
• Binding: Paperback
• Number of pages: 152