Microinjection (Methods and Tools in Biosciences and Medicine) - Softcover

Softcover

ISBN 10: 3764360194 ISBN 13: 9783764360191

Publisher: Birkhäuser, 1999

Synopsis

This manual describes the methodology and applications of microinjection, a powerful technique for transposing material into living cells through needle injection. Information is presented on the techniques principles, the required equipment, preparation of receiving cells and injected material, and the analysis of results. Detailed protocols for a wide range of applications in different cellular systems are included. A large part of the book is also dedicated to the Xenopus laevis oocyte system.

About this title

Synopsis

The technique of needle microinjection has become a prominent experimental approach in biological research. Cellular organelles, DNA and RNA, enzymes, structural proteins, metabolites, ions and antibodies are just some of the molecular and cellular elements that have been transposed from test tubes into living cells by needle injection. This technique is broadly used as a valuable tool for the study of many different cell responses in a variety of systems. This text presents information on the technique of needle microinjection's principles, the required equipment, preparation of receiving cells and injected material and the analysis of the results. It provides detailed protocols for a wide range of important applications and different cellular systems from the fields of cell cycle regulation, signal transduction, study of transcriptional regulations, cytoskeletal functions, secretion and intracellular transport.

Edition details

• Publisher: Birkhäuser
• Publication date: 1999
• Language: English
• ISBN 10: 3764360194
• ISBN 13: 9783764360191
• Binding: Plastic Comb
• Number of pages: 248
• Editor: Lacal Juan Carlos, Perona Rosario, Feramisco James