Comparison of Tooth Decalcification using Microwave & Manual Method: An In Vitro Study - Softcover

Srinivasaiah, Archana; Hegde, Usha; Nitin, Priyanka

Softcover

ISBN 10: 3659835900 ISBN 13: 9783659835902

Publisher: LAP LAMBERT Academic Publishing, 2016

Synopsis

In routine histopathology, decalcification of teeth is an essential and important step during tissue processing which is done by conventional method. Use of microwave oven for decalcification has been tried to decrease the time of reporting. Hence, the present study was attempted to decalcify teeth using microwave method and to compare it with conventional decalcification method. The aim of the study was to compare microwave decalcification with conventional decalcification method with respect to speed of decalcification, preservation of tissue structure and efficacy of staining. A total of 72 single rooted premolars were used for both conventional and microwave decalcification methods. Three different types of decalcifying agents i.e. nitric acid, formic acid and trichloroacetic acid each at 5% and 7% were used. After decalcification, all the teeth were examined macroscopically and microscopically. 5% nitric acid by microwave method proved to be the best decalcifying agent as it was fast, produced very minimal tissue tearing and shrinkage and gave good structural details and staining characteristics.

About this title

About the Author

Dr. Archana Srinivasaiah is presently doing MDS in Oral Pathology & Microbiology from JSS Dental College & Hospital, Mysuru, India. She completed BDS from RV Dental College, Bengaluru, India in 2013. She is member of Indian Association of Oral & Maxillofacial Pathologists (IAOMP). She also got best paper award at 13th National Conference of IAFO.

Edition details

• Publisher: LAP LAMBERT Academic Publishing
• Publication date: 2016
• Language: English
• ISBN 10: 3659835900
• ISBN 13: 9783659835902
• Binding: Paperback
• Number of pages: 96