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Synopsis
Sixteen different mould cultures viz. Aspergillus, Alternaria, Arthroderma, Trichoderma, Fusarium, Penicillium, Rhizopus and Chochliobolus were isolated from the soil samples collected from different localities of Doha (Qatar) by serial dilution method. The preliminary screening of isolates was done by selecting initial colonies showing relatively bigger zones of starch hydrolysis on nutrient agar medium by pour plate method. The isolates were then subjected to secondary screening by submerged fermentation. The product yield was analyzed in dependence of mycelial morphology, biomass level and protein contents. On the basis of kinetic variables, notably A. oryzae IIB-6 was found to be a hyper producer of GGH compared to A. kawachii IIB-2. A noticeable enhancement in enzyme activity of over 30% was observed when process parameters were further optimized using a 2-factorial Plackett-Burman design. The model terms were found to be highly significant (HS, p≤0.05), indicating the potential utility of the culture (dof~3).
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About the Author
Bilqees Fatima has a Bachelor of Science in Medical and Biological Sciences. A Master of Botany with a specialization in Fermentation Technology. A Master of Philosophy in Biotechnology/Biochemistry with a specialization in Protein Purification and a Ph.D in Biotechnology/Molecular Biology with a specialization in Genetic Engineering.PAKISTAN.
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Process optimization for amylase production by Plackett-Burman design
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LAP LAMBERT Academic Publishing Sep 2014, 2014
ISBN 10: 3659611883
ISBN 13: 9783659611889
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Taschenbuch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Sixteen different mould cultures viz. Aspergillus, Alternaria, Arthroderma, Trichoderma, Fusarium, Penicillium, Rhizopus and Chochliobolus were isolated from the soil samples collected from different localities of Doha (Qatar) by serial dilution method. The preliminary screening of isolates was done by selecting initial colonies showing relatively bigger zones of starch hydrolysis on nutrient agar medium by pour plate method. The isolates were then subjected to secondary screening by submerged fermentation. The product yield was analyzed in dependence of mycelial morphology, biomass level and protein contents. On the basis of kinetic variables, notably A. oryzae IIB-6 was found to be a hyper producer of GGH compared to A. kawachii IIB-2. A noticeable enhancement in enzyme activity of over 30% was observed when process parameters were further optimized using a 2-factorial Plackett-Burman design. The model terms were found to be highly significant (HS, p 0.05), indicating the potential utility of the culture (dof~3). 88 pp. Englisch. Seller Inventory # 9783659611889
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Process optimization for amylase production by Plackett-Burman design
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Condition: New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. Autor/Autorin: Fatima BilqeesBilqees Fatima has a Bachelor of Science in Medical and Biological Sciences. A Master of Botany with a specialization in Fermentation Technology. A Master of Philosophy in Biotechnology/Biochemistry with a specializatio. Seller Inventory # 5168266
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Process optimization for amylase production by Plackett-Burman design
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LAP LAMBERT Academic Publishing Sep 2014, 2014
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Taschenbuch. Condition: Neu. This item is printed on demand - Print on Demand Titel. Neuware -Sixteen different mould cultures viz. Aspergillus, Alternaria, Arthroderma, Trichoderma, Fusarium, Penicillium, Rhizopus and Chochliobolus were isolated from the soil samples collected from different localities of Doha (Qatar) by serial dilution method. The preliminary screening of isolates was done by selecting initial colonies showing relatively bigger zones of starch hydrolysis on nutrient agar medium by pour plate method. The isolates were then subjected to secondary screening by submerged fermentation. The product yield was analyzed in dependence of mycelial morphology, biomass level and protein contents. On the basis of kinetic variables, notably A. oryzae IIB-6 was found to be a hyper producer of GGH compared to A. kawachii IIB-2. A noticeable enhancement in enzyme activity of over 30% was observed when process parameters were further optimized using a 2-factorial Plackett-Burman design. The model terms were found to be highly significant (HS, p¿0.05), indicating the potential utility of the culture (dof~3).VDM Verlag, Dudweiler Landstraße 99, 66123 Saarbrücken 88 pp. Englisch. Seller Inventory # 9783659611889
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Process optimization for amylase production by Plackett-Burman design
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Taschenbuch. Condition: Neu. nach der Bestellung gedruckt Neuware - Printed after ordering - Sixteen different mould cultures viz. Aspergillus, Alternaria, Arthroderma, Trichoderma, Fusarium, Penicillium, Rhizopus and Chochliobolus were isolated from the soil samples collected from different localities of Doha (Qatar) by serial dilution method. The preliminary screening of isolates was done by selecting initial colonies showing relatively bigger zones of starch hydrolysis on nutrient agar medium by pour plate method. The isolates were then subjected to secondary screening by submerged fermentation. The product yield was analyzed in dependence of mycelial morphology, biomass level and protein contents. On the basis of kinetic variables, notably A. oryzae IIB-6 was found to be a hyper producer of GGH compared to A. kawachii IIB-2. A noticeable enhancement in enzyme activity of over 30% was observed when process parameters were further optimized using a 2-factorial Plackett-Burman design. The model terms were found to be highly significant (HS, p 0.05), indicating the potential utility of the culture (dof~3). Seller Inventory # 9783659611889
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Process optimization for amylase production by Plackett-Burman design
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Taschenbuch. Condition: Neu. Process optimization for amylase production by Plackett-Burman design | Bilqees Fatima | Taschenbuch | 88 S. | Englisch | 2014 | LAP LAMBERT Academic Publishing | EAN 9783659611889 | Verantwortliche Person für die EU: preigu GmbH & Co. KG, Lengericher Landstr. 19, 49078 Osnabrück, mail[at]preigu[dot]de | Anbieter: preigu. Seller Inventory # 105063685