Synopsis
A new method of in vitro interaction between cell suspension and Cf-elicitor was employed to understand red rot resistance in sugarcane. RT-PCR, differential display (DD)-RT-PCR, RACE-PCR, semi-quantitative RT-PCR and different bioinformatic tools were used to identify and characterize the defense related transcripts. In DD-RT-PCR, many differentially expressed transcripts were identified and functionally grouped. Important candidate genes were identified through RACE-PCR and their functional domains were characterized. Sugarcane chitinase was characterized as a class IV chitinase. Differential accumulation of several transcripts of the flavanoid biosynthetic pathway was established through spatial expression by semi quantitative RT-PCR and this study further confirms the role of sugarcane phytoalexins in red rot resistance. The study has resulted in the generation of large number of TDFs that can be used in future studies in sugarcane-C. falcatum interaction as well as well as other biotic/abiotic stress factors. For the first time, this study established that in vitro interaction between cell suspension and Cf-elicitor mimics molecular interaction in cane tissue and C. falcatum.
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About the Author
Sugarcane Breeding Institute, Coimbatore
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Transcriptomic analysis of defense mechanism in sugarcane
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LAP LAMBERT Academic Publishing Aug 2013, 2013
ISBN 10: 3659415790
ISBN 13: 9783659415791
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Taschenbuch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -A new method of in vitro interaction between cell suspension and Cf-elicitor was employed to understand red rot resistance in sugarcane. RT-PCR, differential display (DD)-RT-PCR, RACE-PCR, semi-quantitative RT-PCR and different bioinformatic tools were used to identify and characterize the defense related transcripts. In DD-RT-PCR, many differentially expressed transcripts were identified and functionally grouped. Important candidate genes were identified through RACE-PCR and their functional domains were characterized. Sugarcane chitinase was characterized as a class IV chitinase. Differential accumulation of several transcripts of the flavanoid biosynthetic pathway was established through spatial expression by semi quantitative RT-PCR and this study further confirms the role of sugarcane phytoalexins in red rot resistance. The study has resulted in the generation of large number of TDFs that can be used in future studies in sugarcane-C. falcatum interaction as well as well as other biotic/abiotic stress factors. For the first time, this study established that in vitro interaction between cell suspension and Cf-elicitor mimics molecular interaction in cane tissue and C. falcatum. 188 pp. Englisch. Seller Inventory # 9783659415791
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Transcriptomic analysis of defense mechanism in sugarcane
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Condition: New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. Autor/Autorin: Rahul P.R.Sugarcane Breeding Institute, CoimbatoreA new method of in vitro interaction between cell suspension and Cf-elicitor was employed to understand red rot resistance in sugarcane. RT-PCR, differential display (DD)-RT-PCR, . Seller Inventory # 5154845
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Transcriptomic Analysis of Defense Mechanism in Sugarcane
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Transcriptomic Analysis of Defense Mechanism in Sugarcane
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Transcriptomic Analysis of Defense Mechanism in Sugarcane
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Transcriptomic analysis of defense mechanism in sugarcane
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Taschenbuch. Condition: Neu. Transcriptomic analysis of defense mechanism in sugarcane | P. R. Rahul (u. a.) | Taschenbuch | 188 S. | Englisch | 2013 | LAP LAMBERT Academic Publishing | EAN 9783659415791 | Verantwortliche Person für die EU: preigu GmbH & Co. KG, Lengericher Landstr. 19, 49078 Osnabrück, mail[at]preigu[dot]de | Anbieter: preigu. Seller Inventory # 105705433
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Transcriptomic analysis of defense mechanism in sugarcane
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LAP LAMBERT Academic Publishing Aug 2013, 2013
ISBN 10: 3659415790
ISBN 13: 9783659415791
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Taschenbuch. Condition: Neu. This item is printed on demand - Print on Demand Titel. Neuware -A new method of in vitro interaction between cell suspension and Cf-elicitor was employed to understand red rot resistance in sugarcane. RT-PCR, differential display (DD)-RT-PCR, RACE-PCR, semi-quantitative RT-PCR and different bioinformatic tools were used to identify and characterize the defense related transcripts. In DD-RT-PCR, many differentially expressed transcripts were identified and functionally grouped. Important candidate genes were identified through RACE-PCR and their functional domains were characterized. Sugarcane chitinase was characterized as a class IV chitinase. Differential accumulation of several transcripts of the flavanoid biosynthetic pathway was established through spatial expression by semi quantitative RT-PCR and this study further confirms the role of sugarcane phytoalexins in red rot resistance. The study has resulted in the generation of large number of TDFs that can be used in future studies in sugarcane-C. falcatum interaction as well as well as other biotic/abiotic stress factors. For the first time, this study established that in vitro interaction between cell suspension and Cf-elicitor mimics molecular interaction in cane tissue and C. falcatum.VDM Verlag, Dudweiler Landstraße 99, 66123 Saarbrücken 188 pp. Englisch. Seller Inventory # 9783659415791
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Transcriptomic analysis of defense mechanism in sugarcane
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LAP LAMBERT Academic Publishing, 2013
ISBN 10: 3659415790
ISBN 13: 9783659415791
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Taschenbuch. Condition: Neu. nach der Bestellung gedruckt Neuware - Printed after ordering - A new method of in vitro interaction between cell suspension and Cf-elicitor was employed to understand red rot resistance in sugarcane. RT-PCR, differential display (DD)-RT-PCR, RACE-PCR, semi-quantitative RT-PCR and different bioinformatic tools were used to identify and characterize the defense related transcripts. In DD-RT-PCR, many differentially expressed transcripts were identified and functionally grouped. Important candidate genes were identified through RACE-PCR and their functional domains were characterized. Sugarcane chitinase was characterized as a class IV chitinase. Differential accumulation of several transcripts of the flavanoid biosynthetic pathway was established through spatial expression by semi quantitative RT-PCR and this study further confirms the role of sugarcane phytoalexins in red rot resistance. The study has resulted in the generation of large number of TDFs that can be used in future studies in sugarcane-C. falcatum interaction as well as well as other biotic/abiotic stress factors. For the first time, this study established that in vitro interaction between cell suspension and Cf-elicitor mimics molecular interaction in cane tissue and C. falcatum. Seller Inventory # 9783659415791