Drought-responsive transcript(s) profiling from the leaves of soybean: Isolation and characterization of drought responsive transcript(s) in Soybean - Softcover

Roy, Priti; Ramesh, S.V.

 
9783659212482: Drought-responsive transcript(s) profiling from the leaves of soybean: Isolation and characterization of drought responsive transcript(s) in Soybean
Softcover
ISBN 10: 3659212482 ISBN 13: 9783659212482
Publisher: LAP LAMBERT Academic Publishing, 2012

Synopsis

Drought responsive genes or transcription factors are important components of any genetic engineering or breeding approach to enhance drought tolerance trait of a crop.Water-deficit stress was imposed on 30 days old seedlings of soybean cultivars Hardee and NRC7.Drought associated physiological parameter like relative water content revealed that water status of the stressed plants is low. Similarly studies on membrane damage disclosed plants under moisture-deficit stress exhibited higher electrolytic leakages, hence greater membrane damage.Total RNA isolated from both the control and stressed plant leaves of cultivars Hardee and NRC-7 was subjected to cDNA synthesis using 3 different anchored primers and thus mRNA population is sub-divided in to 3 different cDNA populations.DDRTPCR using the same anchored primers in combination with eight different arbitrary primers exhibited the transcript profiling of cultivars under moisture deficit stress.The transcript profiling was resolved in denaturing sequencing gel PAGE followed by silver staining so as to identify the differentially expressed transcripts.The book sheds light on drought responsive molecular mechanism of soybean.

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About the Author

Priti Roy received MSc in Biotechnology from Pandit Ravishankar Shukla University Raipur (C.G.);Ramesh SV,Ph.D,working as Scientist (Biotechnology)on soybean improvement at DSR,Indian Council Of Agricultural Research(ICAR)-Indore (M.P.) obtained his doctorate in Plant Biochemistry from Indian Agricultural Research Institute-New Delhi.

"About this title" may belong to another edition of this title.

Publisher
LAP LAMBERT Academic Publishing
Publication date
2012
Language
English
ISBN 10 
3659212482
ISBN 13 
9783659212482
Binding
Paperback
Number of pages
108