Fluorescent Membrane Probes for Apoptosis and Raft Domains: Design, Synthesis and Application - Softcover

Kucherak, Oleksandr

Softcover

ISBN 10: 3659181005 ISBN 13: 9783659181009

Publisher: LAP LAMBERT Academic Publishing, 2012

Synopsis

Fluorescent dyes are of great importance for investigation of biological processes. In 2007 the first ratiometric fluorescent probe for apoptosis detection (F2N12S) has been reported. This book is focused on a development of the improved analogs of F2N12S. Firstly, the systematic studies of F2N12S in model membranes and cells are described for better understanding of its mechanism of response to apoptosis. Secondly, synthesis of new membrane probes is presented. All the molecules are classified into three types according to their sensitivity profiles (sensitive both to the surface charge and to the phase state, only to the surface charge and only to the phase state of lipid membranes). Nile Red derivative NR12S is introduced as a molecular sensor for apoptosis and cholesterol content in cell membrane. Also, the general principle for the design of fluorescent membrane probe is proposed. Finally, two new classes of environment-sensitive fluorophores are presented (on the base of fluorene and chromone units). This book should be interesting for those who are working in the field of biological membranes and on the development of new molecular tools for fluorescence imaging.

About this title

About the Author

Author was born in Kalush, one of regional centers in western Ukraine. After school studies he entered the Kyiv National University (2002), where he got the master degree in organic chemistry (2007). After that author arrived to France where he enjoyed picnics, swimming and making science at the University of Strasbourg.

Edition details

• Publisher: LAP LAMBERT Academic Publishing
• Publication date: 2012
• Language: English
• ISBN 10: 3659181005
• ISBN 13: 9783659181009
• Binding: Paperback
• Number of pages: 156