Cell and Tissue Engineering of Cartilage: Regulation of Chondrocyte by TGF-β and ECM-Proteins - Softcover

Khaghani, Seyed Ali (Behruz)

Softcover

ISBN 10: 3659113069 ISBN 13: 9783659113062

Publisher: LAP LAMBERT Academic Publishing, 2012

Synopsis

Cartilage cell (Chondrocyte) is embedded in the matrix (Lacunae) and has round shape in vivo. The in vitro monolayer culture of primary chondrocyte causes morphological change characterized as dedifferentiation. Transforming growth factor-beta (TGF-β), a cytokine superfamily, regulates cell function, including differentiation and proliferation. The effect of TGF-β1, 2, 3, and their manipulated forms in biological regulation of primary chondrocyte was investigated in this work. A novel method was developed to isolate and purify the primary chondrocytes from knee joint of neonate Sprague-Dawley rat, and the effect of some supplementations such as hyaluronic acid and antibiotics were also investigated to provide the most appropriate condition for in vitro culture of chondrocyte cells.

About this title

About the Author

SA (Behruz) Khaghani, PhD (TE): Studied industry, mechanical and medical engineering in Turkey, Germany and United Kingdom respectively. He received PhD in cell and tissue engineering from University of Bradford and worked in industrial and large organisations for more than 18 years. He is currently a research fellow in cell and tissue engineering.

Edition details

• Publisher: LAP LAMBERT Academic Publishing
• Publication date: 2012
• Language: English
• ISBN 10: 3659113069
• ISBN 13: 9783659113062
• Binding: Paperback
• Number of pages: 212