Engineering of Halogenases towards Synthetic Applications: Increasing the Thermostability and Investigations on a Marine Brominase Bmp5 (BestMasters) - Softcover

Book 47 of 91: BestMasters

Minges, Hannah

 
9783658184094: Engineering of Halogenases towards Synthetic Applications: Increasing the Thermostability and Investigations on a Marine Brominase Bmp5 (BestMasters)
Softcover
ISBN 10: 3658184094 ISBN 13: 9783658184094
Publisher: Springer Spektrum, 2017

Synopsis

Hannah Minges focuses on the investigation of two different FAD-dependent halogenases in order to analyze and improve their applicability for chemoenzymatic approaches in chemistry. Owing to beneficial features, like high selectivity and benign reaction conditions, nature’s toolkit for halogenation provides several advantages, whereas conventional chemical strategies require hazardous reagents and suffer from low selectivity. Therefore, enzymatic halogenation arises as promising alternative in the synthesis of valuable chemicals. One project focuses on the generation of a thermostable variant of the tryptophan halogenase Thal by means of directed evolution. The second project deals with the investigation of the marine halogenase Bmp5. This enzyme is of synthetic interest because it preferably introduces bromine into phenol compounds, whereas chlorination cannot take place. 

"synopsis" may belong to another edition of this title.

About the Author

Hannah Minges, a Ph.D student at Bielefeld University, works on the optimization of tryptophan halogenases by means of directed evolution.

From the Back Cover

Hannah Minges focuses on the investigation of two different FAD-dependent halogenases in order to analyze and improve their applicability for chemoenzymatic approaches in chemistry. Owing to beneficial features, like high selectivity and benign reaction conditions, nature’s toolkit for halogenation provides several advantages, whereas conventional chemical strategies require hazardous reagents and suffer from low selectivity. Therefore, enzymatic halogenation arises as promising alternative in the synthesis of valuable chemicals. One project focuses on the generation of a thermostable variant of the tryptophan halogenase Thal by means of directed evolution. The second project deals with the investigation of the marine halogenase Bmp5. This enzyme is of synthetic interest because it preferably introduces bromine into phenol compounds, whereas chlorination cannot take place. 


Contents
  • Halogenating Enzymes
  • Identification of a Thermostable Thal Variant by Directed Evolution
  • Establishment of the Brominase Bmp5 for Synthetic Application 
Target Groups
Lecturers, students and researchers in biological chemistry and chemistry

The Author
Hannah Minges, a Ph.D student at Bielefeld University, works on the optimization of tryptophan halogenases by means of directed evolution.

"About this title" may belong to another edition of this title.

Publisher
Springer Spektrum
Publication date
2017
Language
English
ISBN 10 
3658184094
ISBN 13 
9783658184094
Binding
Paperback
Edition number
1
Number of pages
128