Protein Structure Analysis: Preparation, Characterization, and Microsequencing - Softcover

Softcover

ISBN 10: 3642592201 ISBN 13: 9783642592201

Publisher: Springer, 2011

Synopsis

I Separation of Proteins.- 1 Purification of Proteins for Sequencing.- 2 Affinity Chromatography of Proteins.- 3 Protein Purification by Aqueous Two-Phase Systems.- 4 Rapid HPLC Separation of Proteins, Peptides, and Amino Acids Using Short Columns Filled with Nonporous Silica-Based Particles.- II Separation of Peptides.- 5 Enzymatic and Chemical Cleavages of Proteins.- 6 Separation of Peptides Using HPLC and TLC Fingerprints.- 7 Microseparation of In Situ Digested Peptides.- 8 Isolation of Peptides for Microsequencing by In-Gel Proteolytic Digestion.- III Manual and Automated Microsequencing Methods.- 9 Automated Microsequencing: Introduction and Overview.- 10 A Manual Method for Protein Sequence Analysis Using the DABITC/PITC Method.- 11 Solid-Phase Sequencing of Peptides and Proteins.- 12 Sequence Analysis of the NH2-Terminally Blocked Proteins Immobilized on PVDF Membranes from Polyacrylamide Gels.- IV Electrophoresis and Blotting of Proteins.- 13 Two-Dimensional Electrophoresis.- 14 Semi-Dry Blotting onto Hydrophobic Membranes.- V Analysis of Amino Acids.- 15 Highly Sensitive Amino Acid Analysis.- 16 Quantitative Analysis of D- and L-Amino Acids by HPLC.- VI Identification of Cysteine Residues and Lipids in Proteins.- 17 Identification of Cysteine Residues and Disulfide Bonds in Proteins.- 18 Lipid Modifications of Proteins.- VII Mass Spectrometry in Peptide and Protein Analysis.- 19 Mass Spectrometry in Peptide and Protein Sequence Analysis.- 20 Carboxy-Terminal Sequencing by Combining Carboxypeptidase Y and P Digestion with Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry.- 21 Crystallization of Biological Macromolecules.

Edition details

• Publisher: Springer
• Publication date: 2011
• Language: English
• ISBN 10: 3642592201
• ISBN 13: 9783642592201
• Binding: Paperback