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Synopsis
This manual introduces the reader to bacic methods used in the isolation, cloning and analysis of genetic material. The protocols include RT-PCR amplification, gene cloning, hybridization analysis and sequencing of nucleic acids, PCR-based site-specific mutagenesis, analysis of protein DNA-specific interaction, cell-free protein synthesis and product electrophoretic and immunological analysis.
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This manual introduces the reader to bacic methods used in the isolation, cloning and analysis of genetic material. The protocols include RT-PCR amplification, gene cloning, hybridization analysis and sequencing of nucleic acids, PCR-based site-specific mutagenesis, analysis of protein DNA-specific interaction, cell-free protein synthesis and product electrophoretic and immunological analysis. Each protocol includes short background information, a detailed description of the necessary materials, step-by-step procedures, a troubleshooting guide and useful practical hints.
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Basic Cloning Procedures (Springer Lab Manuals)
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Basic Cloning Procedures (Paperback)
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Springer-Verlag Berlin and Heidelberg GmbH & Co. KG, Berlin, 2012
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ISBN 13: 9783642489778
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Paperback. Condition: new. Paperback. Experience in laboratory experiments in molecular biology is important to students, researchers and practioners in the biolo- gical and medical sciences. This book is designed to introduce the reader to basic methods used in the isolation, cloning and analysis of genetic material. The text includes theory and fundamental methods in suffi- cient experimental detail which have been successfully used in our studies of viral genome and eukaryotic gene regulation. Spe- cific protocols together with troubleshooting tips are given for RT-PCR amplification, gene cloning, hybridization analysis and sequencing of nucleic acids, PCR-based site-specific mutagene- sis, analysis of protein DNA-specific interaction, cell-free protein synthesis and product electrophoretic and immunological analy- sis. The methods chosen have been approved in several interna- tional Molecular Biology Courses and without doubt can be used as a manual in basic molecular studies of DNA and RNA. The editor is grateful to all the contributors for their coopera- tion and would like to express his thanks to the publishers, espe- cially Dr. J.Lindenborn from Springer-Verlag, for their coopera- tion, patience and assistance during the preparation of this book. Riga, Latvia V. BERZINS Contents Chapter 1 cDNA Synthesis and Cloning .1 JURIS STEINBERGS AND ALEKSANDER TSIMANIS Chapter 2 DNA Sequencing.22 .ERIKS JANKEVICS Chapter 3 RNA In Vitro Synthesis by Phage T7 DNA-Dependent RNA Polymerase.43 .ELITA AVOTA AND NORMUNDS LICIS Chapter 4 PCR-Based Site-Specific Mutagenesis.53 .JANIS KLOVINS AND VALDIS BERZINS Chapter 5 Analysis of Specific Protein-DNA Interactions.68 . . KLOVINS AND VALDIS BERZINS Chapter 5 Analysis of Specific Protein-DNA Interactions. . . . . . . . . 68 . . Shipping may be from multiple locations in the US or from the UK, depending on stock availability. Seller Inventory # 9783642489778
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Basic Cloning Procedures
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Springer Berlin Heidelberg Jul 2012, 2012
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Taschenbuch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This manual introduces the reader to bacic methods used in the isolation, cloning and analysis of genetic material. The protocols include RT-PCR amplification, gene cloning, hybridization analysis and sequencing of nucleic acids, PCR-based site-specific mutagenesis, analysis of protein DNA-specific interaction, cell-free protein synthesis and product electrophoretic and immunological analysis. Each protocol includes short background information, a detailed description of the necessary materials, step-by-step procedures, a troubleshooting guide and useful practical hints. 180 pp. Englisch. Seller Inventory # 9783642489778
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