Dual-focus Fluorescence Correlation Spectroscopy - Softcover

Dertinger, Thomas

Softcover

ISBN 10: 3639027744 ISBN 13: 9783639027747

Publisher: VDM Verlag Dr. Mueller E.K., 2008

Synopsis

This book explains and demonstrates an advancement of conventional Fluorescence Correlation Spectroscopy (FCS), namely Dual-Focus FCS (2fFCS). 2fFCS is a solution based, single-molecule measurement scheme which allows the precise determination of diffusion coefficients of fluorescently labeled molecules (such as proteins, DNA, or other particles) at sub-nanomolar concentrations. In contrast to conventional FCS, where inherent artifacts can lead to substantial errors in the measurement result, 2fFCS allows easy-to-reproduce and artifact-free measurements. This book gives an introduction to 2fFCS, explaining how 2fFCS is able to circumvent FCS inherent artifacts. Beneath the theoretical foundation of 2fFCS also applications will be shown. The reader should be familiar with the basics of fluorescence and microscopy techniques such as confocal microscopy.

Edition details

• Publisher: VDM Verlag Dr. Mueller E.K.
• Publication date: 2008
• Language: English
• ISBN 10: 3639027744
• ISBN 13: 9783639027747
• Binding: Paperback
• Number of pages: 104