Thermal Biosensors Bioactivity Bioaffinity: 64 (Advances in Biochemical Engineering/Biotechnology, 64) - Hardcover

Hardcover

ISBN 10: 3540649670 ISBN 13: 9783540649670

Publisher: Springer-Verlag Berlin and Heidelberg GmbH & Co. K, 1998

Synopsis

The immobilized biocatalyst (IMB) is a key component of biotransformation systems that are used to transform substrates to desired products. The impro- ment of biocatalyst properties has a direct influence on the overall effectiveness of the process based on the biotransformation. The basic catalytic characte- stics of biocatalyst that are followed include kinetic properties, pH optima, stability,and inhibition. The investigation of catalytic properties of immobilized enzymes is still a time consuming procedure and is not always simple. In the 1980s,a major effort was made to standardize the rules by which IMB is char- terized. The Working Party of EFB on immobilized biocatalysts has formul- ed principles of individual methods, among them the requirement of kinetic characterization [1]. It was recommended to use a packed-bed reactor,equipped with temperature control and with infinite flow circulation. The system should be equipped with a post-column unit to measure the time-dependence of the product or substrate concentration [2, 3], the most commonly used analytical methods being spectrophotometry, chemiluminiscence, automatic titration, bioluminiscence, chromatography, polarimetry, and biosensors based on the oxygen electrode. There are two main drawbacks to the application of these methods: 1. The need to vary the analytical principles, depending on the chemical and physical-chemical properties of analytes; 2. In some cases, mainly in the study of hydrolytic enzymes, the natural s- strate must be replaced by an artificial one,that is chromolytic,chromogenic, chemiluminiscent,bioluminiscent,or fluorescent.

About this title

Synopsis

A study of thermal biosensors, bioactivity and bioaffinity. It is the 64th volume in a series covering actual trends in modern biology.

Edition details

• Publisher: Springer-Verlag Berlin and Heidelberg GmbH & Co. K
• Publication date: 1998
• Language: English
• ISBN 10: 3540649670
• ISBN 13: 9783540649670
• Binding: Hardcover
• Number of pages: 248
• Editor: Scheper Thomas