Gradient High Performance Liquid Chromatography of Copolymers and Chromatographic Cross-fractionation - Hardcover

Glockner, Gottfried

Hardcover

ISBN 10: 3540527397 ISBN 13: 9783540527398

Publisher: Springer-Verlag Berlin and Heidelberg GmbH & Co. K, 1991

Synopsis

"The problems involved in separating complex macromolecules require under- standing not only the chromatographic process but also the physicochemical behavior of the solutes." This sentence from the pen of Phyllis R. Brown 1, University of Rhode Island, can certainly be applied to synthetic copolymers whose structure is very complex indeed. Thus it may be forgiven that a book on copolymer HPLC has been written not by a trained chromatographer but by someone from the polymer side. The HPLC of synthetic polymers is often understood to mean only a synonym for size exclusion chromatography. The latter method separates polymers according to the size of the macromolecules and enables the molecular weight distribution of a sample to be evaluated. But as early as 1936, Mark and Saito attempted chromatographic fractionation of cellulose acetate on a charcoal-like adsorbent made from blood. HPLC adsorption chromatography was first applied to copolymer analysis by Teramachi et al. in 1979. Since then, another branch of polymer HPLC has arisen which has the capacity of separating copolymers by composition and enables the chemical composition distribution to be evaluated. The technique requires a suitable elution program and is mainly carried out as gradient elution.

About this title

Synopsis

The combination of size exclusion chromatography and gradient elution HPLC alows the analytical cross-fractionation of polymers. The author has contributed substantially to the development and promotion of this new fast and powerful analytical technique in polymer science. He provides an in-depth treatment of methodology, instrumentation, and applications. Separate chapters deal with the chemical heterogeneity of copolymers, solubility, adsorption processes, mobile and stationary phases, detection and quantitative evaluations in gradient HPLC of polymers. The text should appeal to all polymer scientists, chromatographers and biochemists in industry and academia.

Edition details

• Publisher: Springer-Verlag Berlin and Heidelberg GmbH & Co. K
• Publication date: 1991
• Language: English
• ISBN 10: 3540527397
• ISBN 13: 9783540527398
• Binding: Hardcover
• Number of pages: 226